Biomedical Microdevices

, Volume 15, Issue 5, pp 895–905 | Cite as

A liposome-based ion release impedance sensor for biological detection

  • Gregory L. Damhorst
  • Cartney E. Smith
  • Eric M. Salm
  • Magdalena M. Sobieraj
  • Hengkan Ni
  • Hyunjoon Kong
  • Rashid Bashir
Article

Abstract

Low-cost detection of pathogens and biomolecules at the point-of-care promises to revolutionize medicine through more individualized monitoring and increased accessibility to diagnostics in remote and resource-limited areas. While many approaches to biosensing are still limited by expensive components or inadequate portability, we present here an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet permeabilized for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements, as well as the qualitative detection of viruses as a proof-of-concept toward a portable platform for viral load determination which can be applied broadly to the detection of pathogens and other biomolecules.

Keywords

Impedance sensing Point-of-care Liposomes Microfluidics 

References

  1. T. Chen, D. McIntosh, Y. He, J. Kim, D.A. Tirrell, P. Scherrer, D.B. Fenske, A.P. Sandhu, P.R. Cullis, Mol. Membr. Biol. 21, 385 (2004)CrossRefGoogle Scholar
  2. G. Chen, C.J. Alberts, W. Rodriguez, M. Toner, Anal. Chem. 82, 723 (2010)CrossRefGoogle Scholar
  3. X. Cheng, Y. Liu, D. Irimia, U. Demirci, L. Yang, L. Zamir, W.R. Rodríguez, M. Toner, R. Bashir, Lab. Chip 7, 746 (2007)CrossRefGoogle Scholar
  4. J.T. Connelly, S. Kondapalli, M. Skoupi, J.S.L. Parker, B.J. Kirby, A.J. Baeumner, Anal. Bioanal. Chem. 402, 315 (2012)CrossRefGoogle Scholar
  5. G.L. Damhorst, N.N. Watkins, R. Bashir, IEEE Trans. Bio-med. Eng. 60, 715 (2013)CrossRefGoogle Scholar
  6. A. deMello, D. van Swaay, Lab. Chip. 13, 752 (2012)Google Scholar
  7. K. A. Edwards, O. R. Bolduc, and A. J. Baeumner, Curr. Opin. Chem. Biol. 16, 1 (2012)Google Scholar
  8. D.H. Ekstrand, R.J. Awad, C.F. Källander, J.S. Gronowitz, Biotechnol. Appl. Biochem. 23(Pt 2), 95 (1996)Google Scholar
  9. R. Gómez, R. Bashir, A. Bhunia, Sens. Actuators B: Chem. 86, 198 (2002)CrossRefGoogle Scholar
  10. R. Gómez-sjöberg, D.T. Morisette, R. Bashir, S. Member, J. Microelectromech. Syst. 14, 829 (2005)CrossRefGoogle Scholar
  11. D.D. Ho, Science 272, 1124 (1996)CrossRefGoogle Scholar
  12. M. Hwang, R. Prud’homme, J. Kohn, J. Thomas, Langmuir 17 (2001)Google Scholar
  13. S.A. Kim, J.S. Peacock, J. Immunol. Methods 158, 57 (1993)CrossRefGoogle Scholar
  14. E. Kim, J. Stanton, B. Korber, Nanomedicine 3 (2008)Google Scholar
  15. Y.-G. Kim, S. Moon, D.R. Kuritzkes, U. Demirci, Biosens. Bioelectron. 25, 253 (2009)CrossRefGoogle Scholar
  16. S. Kwakye, V.N. Goral, A.J. Baeumner, Biosens. Bioelectron. 21, 2217 (2006)CrossRefGoogle Scholar
  17. K.-B. Lee, E.-Y. Kim, C.A. Mirkin, S.M. Wolinsky, Nano Lett 4, 1869 (2004)CrossRefGoogle Scholar
  18. S.H. Lee, S.-W. Kim, J.Y. Kang, C.H. Ahn, Lab. Chip 8, 2121 (2008)CrossRefGoogle Scholar
  19. H.H. Lee, M.A. Dineva, Y.L. Chua, A.V. Ritchie, I. Ushiro-Lumb, C.A. Wisniewski, J. Infect. Dis. 201(Suppl), S65 (2010)CrossRefGoogle Scholar
  20. F. Lisdat, D. Schäfer, Anal. Bioanal. Chem. 391, 1555 (2008)CrossRefGoogle Scholar
  21. Q. Liu, B. Boyd, Analyst. 138, 391 (2013)Google Scholar
  22. S.R. Nugen, P.J. Asiello, J.T. Connelly, A.J. Baeumner, Biosens. Bioelectron. 24, 2428 (2009)CrossRefGoogle Scholar
  23. Omega Engineering, Omega Engineering, Inc. 11 (2013)Google Scholar
  24. Z.A. Parpia, R. Elghanian, A. Nabatiyan, D.R. Hardie, D.M. Kelso, JAIDS J. Acquir. Immune Defic. Syndr. 55, 413 (2010)CrossRefGoogle Scholar
  25. H. Shafiee, M. Jahangir, F. Inci, S. Wang, R. B. M. Willenbrecht, F. F. Giguel, A. M. N. Tsibris, D. R. Kuritzkes, and U. Demirci, Small 1 (2013). doi:10.1002/smll.201202195
  26. F. Shen, B. Sun, J.E. Kreutz, E.K. Davydova, W. Du, P.L. Reddy, L.J. Joseph, R.F. Ismagilov, J. Am. Chem. Soc. 133, 17705 (2011)CrossRefGoogle Scholar
  27. S. Tang, I. Hewlett, J. Infect. Dis. 201(Suppl), S59 (2010)CrossRefGoogle Scholar
  28. S. Tang, J. Zhao, J. Storhoff, JAIDS J. Acquir. Immune Defic. Syndr. 46, 231 (2007)CrossRefGoogle Scholar
  29. W. Tang, W.H.A. Chow, Y. Li, H. Kong, Y.-W. Tang, B. Lemieux, J. Infect. Dis. 201(Suppl), S46 (2010)CrossRefGoogle Scholar
  30. S. Tanriverdi, L. Chen, S. Chen, J. Infect. Dis. 201(Suppl), S52 (2010)CrossRefGoogle Scholar
  31. S.-Y. Teh, R. Khnouf, H. Fan, A.P. Lee, Biomicrofluidics 5, 44113 (2011)CrossRefGoogle Scholar
  32. S. Wang, A. Ip, F. Xu, F. F. Giguel, S. Moon, A. Akay, D. R. Kuritzkes, and U. Demirci, sensors, and command, control, communications, and intelligence (C3I) technologies for homeland security and homeland defense IX 7666, 76661H (2010)Google Scholar
  33. S. Wang, M. Esfahani, U.A. Gurkan, F. Inci, D.R. Kuritzkes, U. Demirci, Lab. Chip 12, 1508 (2012)CrossRefGoogle Scholar
  34. Woongjin Chemical Co Ltd, CSM Technical Manual (Seoul, Republic of Korea, 2010), p. 137Google Scholar
  35. N.V. Zaytseva, V.N. Goral, R.A. Montagna, A.J. Baeumner, Lab. Chip 5, 805 (2005a)CrossRefGoogle Scholar
  36. N.V. Zaytseva, R.A. Montagna, A.J. Baeumner, Anal. Chem. 77, 7520 (2005b)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Gregory L. Damhorst
    • 1
    • 2
  • Cartney E. Smith
    • 3
  • Eric M. Salm
    • 1
    • 2
  • Magdalena M. Sobieraj
    • 1
  • Hengkan Ni
    • 4
  • Hyunjoon Kong
    • 3
  • Rashid Bashir
    • 1
    • 2
    • 4
  1. 1.Micro and Nanotechnology LaboratoryUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.Department of BioengineeringUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  3. 3.Department of Chemical and Biomolecular EngineeringUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  4. 4.Department of Electrical and Computer EngineeringUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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