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Biomedical Microdevices

, Volume 14, Issue 6, pp 1019–1025 | Cite as

Development of bacteria-based microrobot using biocompatible poly(ethylene glycol)

  • Sunghoon Cho
  • Sung Jun Park
  • Seong Young Ko
  • Jong-Oh ParkEmail author
  • Sukho ParkEmail author
Article

Abstract

For the development of bacteria-based biomedical microrobot, we propose the fabrication method of biocompatible poly(ethylene glycol) (PEG) microbeads using a cross-junction microfluidic channel. PEG droplets were polymerized by ultraviolet (UV) irradiation to form PEG microbeads of 8.18 ± 3.4 μm diameter in a microfluidic channel. Generally, the bacteria did not attach to the surface of the PEG microbeads because of their hydrophilicity. We modified the selective surface of the PEG microbeads using poly-L-lysine (PLL), promoting attenuated Salmonella typhimurium adhesion using the submerging property of PEG microbeads on agarose gel: the bacteria could thus be attached to the PLL-coated surface region of the PEG microbeads. The selectively PLL-coated PEG microbeads group showed enhanced motility compared with the PLL-uncoated and completely PLL-coated PEG microbeads groups. The selectively PLL-coated PEG microbeads group showed 12.33 and 7.40 times higher average velocities than the PLL-uncoated and completely PLL-coated PEG microbeads groups, respectively. This study verified the successful fabrication of bacteria-based microrobots using PEG microbeads, and the enhanced motility of the microrobots by selective bacteria patterning using agarose gel and PLL.

Keywords

Poly(ethylene glycol) Salmonella typhimurium Patterning Poly-L-lysine Microrobot 

Notes

Acknowledgments

This research was supported by the Future Pioneer R&D program through the National Research Foundation of Korea, funded by the Ministry of Education, Science, and Technology (2012–0001035).

Supplementary material

10544_2012_9704_Fig6_ESM.jpg (7 kb)
S. 1

Microscopic images about migration of bacteria-based microrobot toward 10 mM Aspartic acid-contained capillary tube. (JPEG 6 kb)

10544_2012_9704_MOESM1_ESM.tif (6.9 mb)
High resolution image (TIFF 7095 kb)
10544_2012_9704_Fig7_ESM.jpg (24 kb)
S. 2

Scanning electron microscopy (SEM) (S-4700, Hitachi, Japan) images of the exposed PEG microbead on PDMS. Submerged PEG microbeads were difficult to obtain a fine image of the PEG microbeads on the agarose gel. After the transfer procedure using the PDMS in Fig. 2(c), we have obtained the fine SEM images of the exposed PEG microbeads on the PDMS according to the 1 (S. 2 A and B) and 0.5 % (S. 2 C and D) agarose. (JPEG 23 kb)

10544_2012_9704_MOESM2_ESM.tif (4.7 mb)
High resolution image (TIFF 4845 kb)
ESM 1

(AVI 13706 kb)

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.School of Mechanical Systems EngineeringChonnam National UniversityGwangjuKorea

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