Microactuator device for integrated measurement of epithelium mechanics
- 524 Downloads
Mechanical forces are among important factors that drive cellular function and organization. We present a microfabricated device with on-chip actuation for mechanical testing of single cells. An integrated immersible electrostatic actuator system is demonstrated that applies calibrated forces to cells. We conduct stretching experiments by directly applying forces to epithelial cells adhered to device surfaces functionalized with collagen. We measure mechanical properties including stiffness, hysteresis and visco-elasticity of adherent cells.
KeywordsElectrostatic actuator Force measurement Epithelium Cell mechanics
This work is supported by NSF CAREER award ECS0449499, NIH R01EB006745-01A1 and NSF EFRI (MIKS-1136790). Fabrication was done at the Stanford Nanofabrication Facility, which is supported by NSF under grant ECS 9731293. VM was supported by the Stanford Graduate Fellowship. WJN is supported by National Institutes of Health grant GM35527.
- O. Thoumine, A. Ott, Time scale dependent viscoelastic and contractile regimes in fibroblasts probed by microplate manipulation. J. Cell Sci. 110(17), 2109–2116 (1997)Google Scholar
- Y. Sun, B. Nelson, M.A. Greminger, Investigating protein structure change in the zona pellucida with a microrobotic system. Int. J. Rob. Res. 24(2–3), 211–208 (2004)Google Scholar
- V. Mukundan, P. Ponce, H.E. Butterfield, B.L. Pruitt, Modeling and characterization of electrostatic comb-drive actuators in conducting liquid media. J. Micromechanics Microengineering 19(065008), 9 (2009)Google Scholar
- Y.C. Fung, Biomechanics: Mechanical Properties of Living Tissues. Springer, New York (1993)Google Scholar