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Biomedical Microdevices

, Volume 15, Issue 1, pp 1–8 | Cite as

Bubble Jet agent release cartridge for chemical single cell stimulation

  • N. Wangler
  • M. Welsche
  • M. Blazek
  • M. Blessing
  • M. Vervliet-Scheebaum
  • R. Reski
  • C. Müller
  • H. Reinecke
  • J. Steigert
  • G. Roth
  • R. Zengerle
  • N. Paust
Article

Abstract

We present a new method for the distinct specific chemical stimulation of single cells and small cell clusters within their natural environment. By single-drop release of chemical agents with droplets in size of typical cell diameters (d <30 μm) on-demand micro gradients can be generated for the specific manipulation of single cells. A single channel and a double channel agent release cartridge with integrated fluidic structures and integrated agent reservoirs are shown, tested, and compared in this publication. The single channel setup features a fluidic structure fabricated by anisotropic etching of silicon. To allow for simultaneous release of different agents even though maintaining the same device size, the second type comprises a double channel fluidic structure, fabricated by photolithographic patterning of TMMF. Dispensed droplet volumes are V = 15 pl and V = 10 pl for the silicon and the TMMF based setups, respectively. Utilizing the agent release cartridges, the application in biological assays was demonstrated by hormone-stimulated premature bud formation in Physcomitrella patens and the individual staining of one single L 929 cell within a confluent grown cell culture.

Keywords

Single cell stimulation Drop-on-demand InkJet Agent release Pyscomitrella patens L-929 

Notes

Acknowledgments

We gratefully thank the German Research Foundation (DFG, ZE 527/4 and EXC 294) for financial support of this project. We also appreciate the good cooperation with the Cleanroom Service Center at IMTEK.

Supplementary material

10544_2012_9681_MOESM1_ESM.doc (614 kb)
ESM 1 (DOC 614 kb)

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • N. Wangler
    • 1
    • 2
  • M. Welsche
    • 1
    • 2
  • M. Blazek
    • 1
    • 2
  • M. Blessing
    • 4
  • M. Vervliet-Scheebaum
    • 4
  • R. Reski
    • 1
    • 4
    • 5
  • C. Müller
    • 3
  • H. Reinecke
    • 3
  • J. Steigert
    • 1
  • G. Roth
    • 1
  • R. Zengerle
    • 1
    • 2
  • N. Paust
    • 2
  1. 1.BIOSS – Centre for Biological Signalling StudiesUniversity of FreiburgFreiburgGermany
  2. 2.Laboratory for MEMS Applications, Department of Microsystems Engineering – IMTEKUniversity of FreiburgFreiburgGermany
  3. 3.Laboratory for Process Technology, Department of Microsystems Engineering – IMTEKUniversity of FreiburgFreiburgGermany
  4. 4.Plant Biotechnology, Faculty of BiologyUniversity of FreiburgFreiburgGermany
  5. 5.FRIAS – Freiburg Institute for Advanced StudiesUniversity of FreiburgFreiburgGermany

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