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Biomedical Microdevices

, Volume 14, Issue 2, pp 401–407 | Cite as

Immunocapture of prostate cancer cells by use of anti-PSMA antibodies in microdevices

  • Steven M. Santana
  • He Liu
  • Neil H. Bander
  • Jason P. Gleghorn
  • Brian J. Kirby
Article

Abstract

Patients suffering from cancer can shed tumor cells into the bloodstream, leading to one of the most important mechanisms of metastasis. As such, the capture of these cells is of great interest. Circulating tumor cells are typically extracted from circulation through positive selection with the epithelial cell-adhesion molecule (EpCAM), leading to currently unknown biases when cells are undergoing epithelial-to-mesenchymal transition. For prostate cancer, prostate-specific membrane antigen (PSMA) presents a compelling target for immunocapture, as PSMA levels increase in higher-grade cancers and metastatic disease and are specific to the prostate epithelium. This study uses monoclonal antibodies J591 and J415—antibodies that are highly specific for intact extracellular domains of PSMA on live cells—in microfluidic devices for the capture of LNCaPs, a PSMA-expressing immortalized prostate cancer cell line, over a range of concentrations and shear stresses relevant to immunocapture. Our results show that J591 outperforms J415 and a mix of the two for prostate cancer capture, and that capture performance saturates following incubation with antibody concentrations of 10 micrograms per milliliter.

Keywords

CTC Microfluidic PSMA J591 Circulating tumor cell Prostate cancer 

Abbreviations

PCa

Prostate Cancer

PCTC

Prostate Circulating Tumor Cell

CTC

Circulating Tumor Cell

EpCAM

Epithelial Cell Adhesion Molecule

EMT

Epithelial-to-Mesenchymal Transition

PSMA

Prostate Specific Membrane Antigen

CRPC

Castrate Resistant Prostate Cancer

Notes

Acknowledgements

The work described was partially supported by the Cornell Center on the Microenvironment & Metastasis through Award Number U54CA143876 from the National Cancer Institute, the Cornell NSF GK-12 program and the Cornell-Sloan Fellowship (S.S.). The authors thank LJ Bonassar for use of the plate reader.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Steven M. Santana
    • 1
  • He Liu
    • 2
  • Neil H. Bander
    • 2
  • Jason P. Gleghorn
    • 3
  • Brian J. Kirby
    • 1
    • 4
  1. 1.Sibley School of Mechanical and Aerospace EngineeringCornell UniversityIthacaUSA
  2. 2.Laboratory of Urological OncologyWeill Medical College of Cornell UniversityNew YorkUSA
  3. 3.School of Engineering and Applied Science, Department of Chemical and Biological EngineeringPrinceton UniversityPrincetonUSA
  4. 4.Sibley School of Mechanical and Aerospace EngineeringCornell UniversityIthacaUSA

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