A resettable dynamic microarray device
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This paper describes a simple reusable device that hydrodynamically traps a large number of beads in an array. Guiding pillars allow us to release the trapped beads by simply reversing the flow direction. The trap and reset operations are extremely simple, robust and highly efficient. We analyzed the path of the beads in a microchannel with pillars to optimize the design of the device. We succeeded in arraying hundreds of 100 μm microbeads, subsequently released them in a few minutes, and demonstrated multiple experiments with a single device.
KeywordsDynamic microarray Microbeads handling Microfluidics Reusable device Lab-on-a-chip
We thank Dai Nippon Printing Co., Ltd. for manufacturing devices made of glass-silicon-glass.
Real-time movie showing the clogging of polystyrene microbeads (98.7 μm in diameter) with backward flow in the previous dynamic microarray device (Tan and Takeuchi 2007) (MPG 2430 kb)
Real-time movie showing the trapping of polystyrene microbeads (98.7 μm in diameter) with forward flow. The flow rate ratio of trapping stream and main stream is 2.73 (MPG 2002 kb)
Real-time movie showing the releasing of polystyrene microbeads (98.7 μm in diameter). The flow rate ratio of trapping stream and main stream is 2.18 (MPG 2866 kb)
Real-time movie showing the demonstration of arraying 100 microbeads (98.7 μm in diameter) with forward flow, retrieving single microbead with optically generated bubble (Tan and Takeuchi 2008), and releasing the trapped microbeads with backward flow in a single device. The flow rate ratio of trapping stream and main stream is 2.18. The device is made of glass and silicon with aluminum pads at the outlets of the trapping spots for generating bubbles, which is fabricated through the foundry service of Dai Nippon Printing Co., Ltd. (MPG 8538 kb)
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