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Biomedical Microdevices

, Volume 13, Issue 6, pp 1033–1042 | Cite as

Purification of cell subpopulations via immiscible filtration assisted by surface tension (IFAST)

  • Scott M. Berry
  • Lindsay N. Strotman
  • Jessica D. Kueck
  • Elaine T. Alarid
  • David J. BeebeEmail author
Article

Abstract

The selective isolation of a sub-population of cells from a larger, mixed population is a critical preparatory process to many biomedical assays. Here, we present a new cell isolation platform with a unique set of advantages over existing devices. Our technology, termed Immiscible Filtration Assisted by Surface Tension, exploits physical phenomena associated with the microscale to establish fluidic barriers composed of immiscible liquids. By attaching magnetically-responsive particles to a target cell population via immunocapture, we can selectively transport this population across the immiscible barrier and into a separate aqueous solution. The high interfacial energy associated with the immiscible phase / aqueous phase boundaries prevents unwanted cells or other contaminants from inadvertently crossing the immiscible phase. We have demonstrated, using fluorescent particles, stromal cells, and whole blood as “background”, that we can successfully isolate ~70% of a target breast cancer cell population with an average purity of >80%. Increased purity was obtained by coupling two immiscible barriers in series, a modification that only slightly increases operational complexity. Furthermore, several samples can be processed in parallel batches in a near-instantaneous manner without the requirement of any washing, which can cause dilution (negative selection) or significant uncontrolled loss (positive selection) of target cells. Finally, cells were observed to remain viable and proliferative following traverse through the immiscible phase, indicating that this process is suitable for a variety of downstream assays, including those requiring intact living cells.

Keywords

Cell sorting Microfluidics Immiscible phase filtration IFAST 

Notes

Acknowledgements

This work was funded by the University of Wisconsin Stem Cell and Regenerative Medicine Center, the Walter H. Coulter Translational Research Partnership, National Institutes of Health Grant #5R33CA137673, and Department of Defense BCRP Concept Award #W81XWH-08-1-0525. J.D. Kueck is a Mary Engsberg Fellow. A portion of this work was funded by DHS Science and Technology Assistance Agreement No. 2007-ST-061-000003 awarded by the U.S. Department of Homeland Security. It has not been formally reviewed by DHS. The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security. The Department of Homeland Security does not endorse any products or commercial services mentioned in this publication.

Supplementary material

10544_2011_9573_Fig6_ESM.jpg (21 kb)
Fig. S-1

The average image fluorescence of MCF-7 cells that constitutively express GFP increases linearly (R2 = 0.99) with increasing cell concentration (JPEG 20 kb)

10544_2011_9573_MOESM1_ESM.tif (36 kb)
High resolution image (TIFF 36 kb)
Supplementary Video 1

Complete loading and operation of array of five IFAST devices (MP4 14413 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Scott M. Berry
    • 1
    • 2
  • Lindsay N. Strotman
    • 1
    • 2
  • Jessica D. Kueck
    • 2
    • 3
  • Elaine T. Alarid
    • 2
    • 3
  • David J. Beebe
    • 1
    • 2
    Email author
  1. 1.Department of Biomedical EngineeringUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Carbone Cancer Center and Wisconsin Institutes for Medical ResearchUniversity of Wisconsin-MadisonMadisonUSA
  3. 3.Department of OncologyUniversity of Wisconsin-MadisonMadisonUSA

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