Biomedical Microdevices

, Volume 13, Issue 4, pp 759–767

Novel disposable biochip platform employing supercritical angle fluorescence for enhanced fluorescence collection

  • Duncan Hill
  • Barry McDonnell
  • Stephen Hearty
  • Lourdes Basabe-Desmonts
  • Robert Blue
  • Michal Trnavsky
  • Colm McAtamney
  • Richard O’Kennedy
  • Brian D. MacCraith
Article

Abstract

This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a planar substrate. The optical elements are based on supercritical angle fluorescence (SAF) which provides substantial enhancement of the fluorescence collection efficiency but also confines the fluorescence detection volume strictly to the immediate proximity of the biochip surface, thereby having the potential to discriminate against background fluorescence from the analyte solution. An optical reader is also described that enables interrogation and fluorescence collection from the nine optical elements on the chip. The sensitivity of the system was determined with a biotin-avidin assay while its clinical utility was demonstrated in an assay for C-reactive protein (CRP), an inflammation marker.

Keywords

Lab on a chip Supercritical angle fluorescence Immunoassay Cardiac marker 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Duncan Hill
    • 1
  • Barry McDonnell
    • 1
  • Stephen Hearty
    • 1
  • Lourdes Basabe-Desmonts
    • 1
  • Robert Blue
    • 1
  • Michal Trnavsky
    • 1
  • Colm McAtamney
    • 1
  • Richard O’Kennedy
    • 1
  • Brian D. MacCraith
    • 1
  1. 1.Biomedical Diagnostics InstituteDublin City UniversityDublin 9Ireland

Personalised recommendations