Biomedical Microdevices

, Volume 13, Issue 4, pp 753–758 | Cite as

Microfluidic array for three-dimensional perfusion culture of human mammary epithelial cells

  • Shin-Yi Cindy Chen
  • Paul J. Hung
  • Philip J. Lee
Article

Abstract

The ability to culture cells in three dimensional extracellular matrix (3D ECM) has proven to be an important tool for laboratory biology. Here, we demonstrate a microfluidic perfusion array on a 96-well plate format capable of long term 3D ECM culture within biomimetic microchambers. The array consists of 32 independent flow units, each with a 4 μl open-top culture chamber, and 350 μl inlet and outlet wells. Perfusion is generated using gravity and surface tension forces, allowing the array to be operated without any external pumps. MCF-10A mammary epithelial cells cultured in Matrigel in the microfluidic array exhibit acinus morphology over 9 days consistent with previous literature. We further demonstrated the application of the microfluidic array for in vitro anti-cancer drug screening.

Keywords

Microfluidics 3D cell culture Mammary epithelial cells MCF-10A 

Supplementary material

10544_2011_9545_MOESM1_ESM.pdf (102 kb)
Supplementary Figure 1 Culture configurations in the microfluidic 3D ECM array. MCF-10A cells cultured for 4 days in the microfluidic chamber with live/dead staining in 2D, 3D overlayed and 3D embedded. (PDF 102 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Shin-Yi Cindy Chen
    • 1
  • Paul J. Hung
    • 1
  • Philip J. Lee
    • 1
  1. 1.CellASIC CorporationHaywardUSA

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