Lectin-mediated microfluidic capture and release of leukemic lymphocytes from whole blood
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Lectins are a group of proteins that bind specifically and reversibly to mono- and oligosaccharide carbohydrate structures that are present on the surfaces of mammalian cells. The use of lectins as capture agents in microfluidic channels was examined with a focus on cells associated with T and B lymphocytic leukemia. In addition to examining the adhesion of Jurkat T and Raji B lymphocytes to a broad panel of lectins, this work also examined the capture of these cells from whole blood. Captured T and B lymphocytes were eluted from the microfluidic devices with a solution of the lectin’s inhibiting sugar. The capture and release steps were accomplished in under 1 h. The significance of this work lies within the realm of low-cost capture of abundant target cells with non-stimulatory elution capability.
KeywordsMicrofluidics Lectins Cell capture Cell adhesion Blood Leukemia
We gratefully acknowledge financial support from internal research funds provided to SKM by Northeastern University. This work was also supported by the National Cancer Institute (U01-CA128427-01) and by the Korean Research WCU Grant R31-2008-000-10086-0.
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