Biomedical Microdevices

, Volume 13, Issue 2, pp 383–392 | Cite as

Efficient large volume electroporation of dendritic cells through micrometer scale manipulation of flow in a disposable polymer chip

  • David Selmeczi
  • Thomas S. Hansen
  • Özcan Met
  • Inge Marie Svane
  • Niels B. Larsen
Article

Abstract

We present a hybrid chip of polymer and stainless steel designed for high-throughput continuous electroporation of cells in suspension. The chip is constructed with two parallel stainless steel mesh electrodes oriented perpendicular to the liquid flow. The relatively high hydrodynamic resistance of the micrometer sized holes in the meshes compared to the main channel enforces an almost homogeneous flow velocity between the meshes. Thereby, very uniform electroporation of the cells can be accomplished. Successful electroporation of 20 million human dendritic cells with mRNA is demonstrated. The performance of the chip is similar to that of the traditional electroporation cuvette, but without an upper limit on the number of cells to be electroporated. The device is constructed with two female Luer parts and can easily be integrated with other microfluidic components. Furthermore it is fabricated from injection molded polymer parts and commercially available stainless steel mesh, making it suitable for inexpensive mass production.

Keywords

Electroporation Cancer immunotherapy Transfection Microfluidics Lab on a chip 

Supplementary material

10544_2010_9507_MOESM1_ESM.doc (1014 kb)
ESM 1(DOC 0.99 MB)

References

  1. K.O. Andresen, M. Hansen, M. Matschuk, S.T. Jepsen, H.S. Sorensen, P. Utko, D. Selmeczi, T.S. Hansen, N.B. Larsen, N. Rozlosnik, R. Taboryski, J Micromech Microeng 20, 055010 (2010)CrossRefGoogle Scholar
  2. J. Banchereau, R.M. Steinman, Nature 392, 245 (1998)CrossRefGoogle Scholar
  3. J.N. Blattman, P.D. Greenberg, Science 305, 200 (2004)CrossRefGoogle Scholar
  4. T. Geng, Y. Zhan, H.Y. Wang, S.R. Witting, K.G. Cornetta, C. Lu, J. Control. Release (2010)Google Scholar
  5. P. Guermonprez, J. Valladeau, L. Zitvogel, C. Thery, S. Amigorena, Annu Rev Immunol 20, 621 (2002)CrossRefGoogle Scholar
  6. E.G. Guignet, T. Meyer, Nat Meth 5, 393 (2008)CrossRefGoogle Scholar
  7. N.J. Hallab, S. Anderson, M. Caicedo, A. Brasher, K. Mikecz, J.J. Jacobs, J Biomed Mater Res Part A 74A, 124 (2005)CrossRefGoogle Scholar
  8. T.S. Hansen, D. Selmeczi, N.B. Larsen, J Micromech Microeng 20, 015020 (2010)CrossRefGoogle Scholar
  9. A. Hosoi, Y. Takeda, K. Sakuta, S. Ueha, M. Kurachi, K. Kimura, R. Maekawa, K. Kakimi, Biochem Biophys Res Commun 371, 242 (2008)CrossRefGoogle Scholar
  10. K.S. Huang, Y.C. Lin, K.C. Su, H.Y. Chen, Biomed Microdevices 9, 761 (2007a)CrossRefGoogle Scholar
  11. K. Huang, Y. Lin, C. Su, C. Fang, Lab Chip 7, 86 (2007b)CrossRefGoogle Scholar
  12. T. Jain, J. Muthuswamy, Lab Chip 7, 1004 (2007)CrossRefGoogle Scholar
  13. M.F. Kalady, M.W. Onaitis, K.M. Padilla, S. Emani, D.S. Tyler, S.K. Pruitt, J Surg Res 105, 17 (2002)CrossRefGoogle Scholar
  14. P. Kalinski, J. Urban, R. Narang, E. Berk, E. Wieckowski, R. Muthuswamy, Future Oncol 5, 379 (2009)CrossRefGoogle Scholar
  15. M. Khine, A. Lau, C. Ionescu-Zanetti, J. Seo, L.P. Lee, Lab Chip 5, 38 (2005)CrossRefGoogle Scholar
  16. M. Khine, C. Ionescu-Zanetti, A. Blatz, L. Wang, L.P. Lee, Lab Chip 7, 457 (2007)CrossRefGoogle Scholar
  17. A. Lanzavecchia, F. Sallusto, Cell 106, 263 (2001)CrossRefGoogle Scholar
  18. W.G. Lee, U. Demirci, A. Khademhosseini, Integr Biol (Camb) 1, 242 (2009)CrossRefGoogle Scholar
  19. Y.C. Lin, M. Li, C.C. Wu, Lab Chip 4, 104 (2004)CrossRefGoogle Scholar
  20. S.N. Markovic, A.B. Dietz, C.W. Greiner, M.L. Maas, G.W. Butler, D.J. Padley, P.A. Bulur, J.B. Allred, E.T. Creagan, J.N. Ingle, D.A. Gastineau, S. Vuk-Pavlovic, J Transl Med 4, 35 (2006)CrossRefGoogle Scholar
  21. O. Met, J. Eriksen, I.M. Svane, Mol Biotechnol 40, 151 (2008)CrossRefGoogle Scholar
  22. O. Met, E. Balslev, H. Flyger, I.M. Svane, Breast Cancer Res. Treat. (2010)Google Scholar
  23. P. Ponsaerts, V.F. Van Tendeloo, N. Cools, A. Van Driessche, F. Lardon, G. Nijs, M. Lenjou, G. Mertens, C. Van Broeckhoven, D.R. Van Bockstaele, Z.N. Berneman, Leukemia 16, 1324 (2002)CrossRefGoogle Scholar
  24. D.A. Puleo, W.W. Huh, J Appl Biomater 6, 109 (1995)CrossRefGoogle Scholar
  25. D. Ridgway, Cancer Invest 21, 873 (2003)CrossRefGoogle Scholar
  26. S. Saeboe-Larssen, E. Fossberg, G. Gaudernack, J Immunol Methods 259, 191 (2002)CrossRefGoogle Scholar
  27. N. Schaft, J. Dorrie, P. Thumann, V.E. Beck, I. Muller, E.S. Schultz, E. Kampgen, D. Dieckmann, G. Schuler, J Immunol 174, 3087 (2005)Google Scholar
  28. D.H. Schuurhuis, P. Verdijk, G. Schreibelt, E.H. Aarntzen, N. Scharenborg, A. de Boer, M.W. van de Rakt, M. Kerkhoff, M.J. Gerritsen, F. Eijckeler, J.J. Bonenkamp, W. Blokx, J.H. van Krieken, O.C. Boerman, W.J. Oyen, C.J. Punt, C.G. Figdor, G.J. Adema, I.J. de Vries, Cancer Res 69, 2927 (2009)CrossRefGoogle Scholar
  29. E.L. Smits, S. Anguille, N. Cools, Z.N. Berneman, V.F. Van Tendeloo, Hum Gene Ther 20, 1106 (2009)CrossRefGoogle Scholar
  30. P.J. Tacken, I.J. de Vries, R. Torensma, C.G. Figdor, Nat Rev Immunol 7, 790 (2007)CrossRefGoogle Scholar
  31. S. Tuyaerts, A. Michiels, J. Corthals, A. Bonehill, C. Heirman, C. de Greef, S.M. Noppe, K. Thielemans, Cancer Gene Ther 10, 696 (2003)CrossRefGoogle Scholar
  32. A. Valero, J.N. Post, J.W. van Nieuwkasteele, P.M. ter Braak, W. Kruijer, A. van den Berg, Lab Chip 8, 62 (2008)CrossRefGoogle Scholar
  33. V.F. Van Tendeloo, P. Ponsaerts, Z.N. Berneman, Curr Opin Mol Ther 9, 423 (2007)Google Scholar
  34. S.A. Yakovenko, Electroporation Chamber. 10/872242 (2005)Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • David Selmeczi
    • 1
  • Thomas S. Hansen
    • 1
  • Özcan Met
    • 2
  • Inge Marie Svane
    • 2
  • Niels B. Larsen
    • 1
  1. 1.Department of Micro- and NanotechnologyTechnical University of DenmarkKgs. LyngbyDenmark
  2. 2.Center for Cancer Immune Therapy (CCIT), Department of Hematology; and Department of OncologyHerlev University HospitalHerlevDenmark

Personalised recommendations