Microfabricated glass devices for rapid single cell immobilization in mouse zygote microinjection
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This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that the device is effective for rapid cell immobilization and does not produce negative effect on embryonic development.
KeywordsSingle cell immobilization Cell holding device Microfabrication Cell microinjection
This work was supported by the Natural Sciences and Engineering Research Council of Canada, the Ontario Ministry of Research and Innovation, and the Ontario Centers of Excellence. We also acknowledge the financial support from the Canada Research Chairs program to YS, and the Ontario Graduate Scholarship program to XL.
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