Biomedical Microdevices

, 11:997

Contactless dielectrophoresis: a new technique for cell manipulation

  • Hadi Shafiee
  • John L. Caldwell
  • Michael B. Sano
  • Rafael V. Davalos
Article

DOI: 10.1007/s10544-009-9317-5

Cite this article as:
Shafiee, H., Caldwell, J.L., Sano, M.B. et al. Biomed Microdevices (2009) 11: 997. doi:10.1007/s10544-009-9317-5

Abstract

Dielectrophoresis (DEP) has become a promising technique to separate and identify cells and microparticles suspended in a medium based on their size or electrical properties. Presented herein is a new technique to provide the non-uniform electric field required for DEP that does not require electrodes to contact the sample fluid. In our method, electrodes are capacitively-coupled to a fluidic channel through dielectric barriers; the application of a high-frequency electric field to these electrodes then induces an electric field in the channel. This technique combines the cell manipulation abilities of traditional DEP with the ease of fabrication found in insulator-based technologies. A microfluidic device was fabricated based on this principle to determine the feasibility of cell manipulations through contactless DEP (cDEP). We were able to demonstrate cell responses unique to the DEP effect in three separate cell lines. These results illustrate the potential for this technique to identify cells through their electrical properties without fear of contamination from electrodes.

Keywords

BioMEMS Biochip Dielectrophoretic Microfluidics Sample handling Electrorotation Sample preparation 

Supplementary material

video 1

(MPG 37262 kb)

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Hadi Shafiee
    • 1
    • 4
  • John L. Caldwell
    • 2
    • 4
  • Michael B. Sano
    • 1
    • 4
  • Rafael V. Davalos
    • 1
    • 3
    • 4
  1. 1.Engineering Science and Mechanics DepartmentVirginia TechBlacksburgUSA
  2. 2.Bradley Department of Electrical and Computer EngineeringVirginia TechBlacksburgUSA
  3. 3.School of Biomedical Engineering & SciencesVirginia Tech—Wake Forest UniversityBlacksburgUSA
  4. 4.Cellular ElectroMechanical Systems (CEMS) Laboratory, Institute for Critical Technology and Applied Science (ICTAS)Virginia TechBlacksburgUSA

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