Biomedical Microdevices

, Volume 11, Issue 4, pp 883–892 | Cite as

Microdevice for the isolation and enumeration of cancer cells from blood

  • Swee Jin Tan
  • Levent Yobas
  • Gabriel Yew Hoe Lee
  • Choon Nam Ong
  • Chwee Teck Lim
Article

Abstract

Cancer metastasis is the main attribute to cancer-related deaths. Furthermore, clinical reports have shown a strong correlation between the disease development and number of circulating tumor cells (CTCs) in the peripheral blood of cancer patients. Here, we present a label-free microdevice capable of isolating cancer cells from whole blood via their distinctively different physical properties such as deformability and size. The isolation efficiency is at least 80% for tests performed on breast and colon cancer cells. Viable isolated cells are also obtained which may give further insights to the understanding of the metastatic process. Contrasting with conventional biochemical techniques, the uniqueness of this microdevice lies in the mechanistic and efficient means of isolating viable cancer cells in blood. The microdevice has the potential to be used for routine monitoring of cancer development and cancer therapy in a clinical setting.

Keywords

Circulating tumor cells (CTCs) Microfluidic device Cancer cell isolation Metastasis Physical separation Biomechanical properties Cell mechanics 

Supplementary material

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Movie 4 (MPG 5.14 mb)

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Swee Jin Tan
    • 1
    • 2
  • Levent Yobas
    • 1
  • Gabriel Yew Hoe Lee
    • 3
  • Choon Nam Ong
    • 4
  • Chwee Teck Lim
    • 2
    • 3
    • 5
  1. 1.Institute of MicroelectronicsA*STAR (Agency for Science, Technology and Research)SingaporeSingapore
  2. 2.Graduate School for Integrative Sciences and EngineeringNational University of SingaporeSingaporeSingapore
  3. 3.Singapore-MIT AllianceSingaporeSingapore
  4. 4.Department of Epidemiology and Public HealthNational University of SingaporeSingaporeSingapore
  5. 5.Division of Bioengineering and Department of Mechanical EngineeringNational University of SingaporeSingaporeSingapore

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