Biomedical Microdevices

, Volume 11, Issue 3, pp 671–678 | Cite as

Sample preparation module for bacterial lysis and isolation of DNA from human urine

  • M. Dominika Kulinski
  • Madhumita Mahalanabis
  • Sara Gillers
  • Jane Y. Zhang
  • Satish Singh
  • Catherine M. KlapperichEmail author


Silica impregnated polymer monolithic columns may provide a simple method for lysing and extracting DNA from bacteria inside of microfluidic chips. Here we use Escherichia coli as a test organism for a point of care thermoplastic microfluidic module designed to take in a urine sample, mix it with lysis buffer, and perform a hybrid chemical/mechanical lysis and solid phase extraction of nucleic acids from the sample. To demonstrate proof-of-concept, we doped human hematuric urine samples with E. coli at concentrations ranging from 101–105 colony-forming units/mL (CFU/mL) to simulate patient samples. We then performed on-chip lysis and DNA extraction. The bacterial DNA was amplified using real-time PCR demonstrating lysis and isolation down to 101 CFU/mL. Results were comparable to a commercial kit at higher concentrations and performed better at recovering DNA at lower concentrations.


Urine Gram-negative Bacteria Lysis PCR Urinary tract infection Microfluidic Thermoplastic Cyclic polyolefins 



We thank the Wallace H. Coulter Foundation for funding this research and H. Muayad for help with image analysis.


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • M. Dominika Kulinski
    • 1
  • Madhumita Mahalanabis
    • 3
  • Sara Gillers
    • 2
  • Jane Y. Zhang
    • 3
  • Satish Singh
    • 2
  • Catherine M. Klapperich
    • 1
    • 3
    Email author
  1. 1.Department of Mechanical EngineeringBoston UniversityBostonUSA
  2. 2.Boston University Medical CenterBostonUSA
  3. 3.Department of Biomedical EngineeringBoston UniversityBostonUSA

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