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Biomedical Microdevices

, Volume 11, Issue 1, pp 103–115 | Cite as

Biomimetic hydrogels for biosensor implant biocompatibility: electrochemical characterization using micro-disc electrode arrays (MDEAs)

  • Gusphyl Justin
  • Stephen Finley
  • Abdur Rub Abdur Rahman
  • Anthony Guiseppi-Elie
Article

Abstract

Our interest is in the development of engineered microdevices for continuous remote monitoring of intramuscular lactate, glucose, pH and temperature during post-traumatic hemorrhaging. Two important design considerations in the development of such devices for in vivo diagnostics are discussed; the utility of micro-disc electrode arrays (MDEAs) for electrochemical biosensing and the application of biomimetic, bioactive poly(HEMA)-based hydrogel composites for implant biocompatibility. A poly(HEMA)-based hydrogel membrane containing polyethylene glycol (PEG) was UV cross-linked with tetraethyleneglycol diacrylate following application to MDEAs (50 μm discs) and to 250 μm diameter gold electrodes within 8-well culture ware. Cyclic voltammetry (CV) of the MDEAs revealed a reduction in the apparent diffusion coefficient of ferrocenemonocarboxylic acid (FcCO2H), from 6.68 × 10−5 to 6.74 × 10−6 cm2/s for the uncoated and 6 μm thick hydrogel coated devices, respectively. Single frequency (4 kHz) temporal impedance measurements of the hydrogels in the 8-well culture ware showed a reversible 5% change in the absolute impedance of the hydrogels when exposed to a pH change between 6.1 to 7.2 and a 20% drop between pH 6.1 and 8.8.

Keywords

Biotransducers Implantable biosensors Integrated circuits Arrays Electroanalysis 

Notes

Funding

This work was supported by the US Department of Defense (DoDPRMRP) grant PR023081/DAMD17-03-1-0172 and by the Consortium of the Clemson University Center for Bioelectronics, Biosensors and Biochips (C3B).

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Gusphyl Justin
    • 1
  • Stephen Finley
    • 1
    • 2
  • Abdur Rub Abdur Rahman
    • 1
  • Anthony Guiseppi-Elie
    • 1
    • 2
    • 3
  1. 1.Center for Bioelectronics, Biosensors and Biochips (C3B)Clemson University Advanced Materials CenterAndersonUSA
  2. 2.Department of Chemical and Biomolecular EngineeringClemson UniversityClemsonUSA
  3. 3.Department of BioengineeringClemson UniversityClemsonUSA

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