Towards an implantable biochip for glucose and lactate monitoring using microdisc electrode arrays (MDEAs)
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A complete electrochemical cell-on-a-chip that uses the MicroDisc Electrode Array (MDEA) working electrode (WE) design was evaluated for eventual intramuscular implantation for the continuous amperometric monitoring of glucose and lactate in an animal trauma model. The microfabricated ECC MDEA5037 comprises two discrete electrochemical cells-on-a-chip (ECCs), each with a reference, counter, and MDEA working electrode. Each MDEA comprises 37 microdiscs (diameter = 50 µm) arranged in a Hexagonal Closed Packed (HCP) arrangement with a center to center distance (d) of 100 µm. Cyclic Voltammetry (CV) and Electrical Impendence Spectroscopy (EIS) reveals that this device scales in its interfacial properties with a corresponding MDEA 050 device that comprises 5,184 microdiscs. Parallel development of miniaturized mixed-signal integrated electronics for wireless reprogramming, data acquisition and communication addresses the key issues involved in developing measurement electronics, AD/DA processing, power management and telemetry for implantable amperometric biosensors. A generalized electronics platform based on the Texas Instruments TI NC01101 chip has been developed that may be readily applied to many types of biotransducers with minor modifications.
KeywordsBiotransducers Implantable biosensors Microarrays Integrated biochips
This work was supported by the US Department of Defense (DoDPRMRP) grant PR023081/DAMD17-03-1-0172 and by the Consortium of the Clemson University Center for Bioelectronics, Biosensors and Biochips. The authors acknowledge work to order contributions of Peter Hansen at Telesensors.
- J. Aguilo, J. Millan, R. Villa, Micro and nano technologies in medical applications: a challenge, in Semiconductor Conference, 2001. CAS 2001 Proceedings. International, vol. 1, 247–255 (2001)Google Scholar
- M.J. Madou, Fundamentals of Microfabrication (CRC, Boca Raton, Fla, 1997)Google Scholar
- P. Rai-Choudhury, Handbook of Microlithography, Micromachining, and Microfabrication: Microlithography (Society of Photo Optical, California, 1997)Google Scholar
- J. Wang, Glucose Biosensors: 40 Years of Advances and Challenges Electroanalysis 13, 983 (2001)doi: 10.1002/1521-4109(200108)13:12<983::AID-ELAN983>3.0.CO;2-# CrossRefGoogle Scholar
- F.F. Zhang, Q. Wan, X.L. Wang, Z.D. Sun, Z.Q. Zhu, Y.Z. Xian et al., Amperometric sensor based on ferrocene-doped silica nanoparticles as an electron transfer mediator for the determination of glucose in rat brain coupled to in vivo microdialysis J. Electroanal. Chem. 571, 133–138 (2004)doi: 10.1016/j.jelechem.2004.04.019 CrossRefGoogle Scholar