Biomedical Microdevices

, 10:807

A high-yield method for generating mass-transfer gradients in elastomer microfluidics using impermeable capillaries

  • Mike Pinelis
  • Leonid Shamban
  • Andreja Jovic
  • Michel M. Maharbiz
Article

Abstract

We demonstrated a robust, inexpensive method for fabricating high-throughput gas gradient generation devices which combined classic mass-transfer limited techniques with the versatility of elastomer microfluidics. The method allowed for dozens of replicate mass-transfer gradient experiments per day, including fabrication and assembly of devices. We demonstrated how our devices can be interfaced with microfluidics and how gradient parameters can be varied. In this work, we applied the method to characterize gradients of pH and cell viability generated in mass-transfer limited cultures of HeLa cells and observed the morphology of differentiating C2C12 myoblasts in an oxygen gradient.

Keywords

Oxygen Gradient Cell Culture Gas High Throughput Chemotaxis 

Supplementary material

10544_2008_9195_MOESM1_ESM.doc (5.4 mb)
Supplemental Information(DOC 5.38 MB)

References

  1. J.W. Allen, S.N. Bhatia, Biotechnol. Bioeng. 82, 253–262 (2003)CrossRefGoogle Scholar
  2. J.M. Brown, W.R. Wilson, Nat. Rev. Cancer 4, 437–447 (2004)CrossRefGoogle Scholar
  3. S.I. Bibikov, R. Biran, K.E. Rudd, J.S. Parkinson, J. Bacteriol. 179, 4075–4079 (1997)Google Scholar
  4. S.I. Bibikov, L.A. Barnes, Y. Gitin, J.S. Parkinson, Proc. Natl. Acad. Sci. U.S.A. 97, 5830–5835 (2000)CrossRefGoogle Scholar
  5. B.H. Chueh et al., Anal. Chem. 79, 3504–3508 (2007)CrossRefGoogle Scholar
  6. B.G. Chung et al., Lab Chip 5, 401–406 (2005)CrossRefGoogle Scholar
  7. D.M. Cochran et al., Ann. Biomed. Eng. 34, 1247–1258 (2006)CrossRefGoogle Scholar
  8. M. Csete, Anesthesiol. Clin. North Am. 16, 201–210 (1998)CrossRefGoogle Scholar
  9. G. Helmlinger et al., Nature 405, 139–141 (2000)CrossRefGoogle Scholar
  10. G. Helmlinger, A. Schell, M. Dellian, N.S. Forbes, R.K. Jain, Clin. Cancer Res. 8, 1284–1291 (2002)Google Scholar
  11. T.S. Hussein et al., J. Cell Sci. 118, 5257–5268 (2005)CrossRefGoogle Scholar
  12. M. Hülskamp, C. Pfeifle, D. Tautz, Nature 346, 577–580 (1990)CrossRefGoogle Scholar
  13. D. Irimia, D. Geba, M. Toner, Anal. Chem. 78, 3472–3477 (2006)CrossRefGoogle Scholar
  14. N.L. Jeon et al., Nat. Biotechnol. 20, 826–830 (2002)MathSciNetGoogle Scholar
  15. A. Levchenko, P.A. Iglesias, Biophys. J. 82, 50–63 (2002)CrossRefGoogle Scholar
  16. X. Li, L.L. Zhu, X.P. Chen, M. Fan, Med. Hypotheses 69, 629–636 (2007)CrossRefGoogle Scholar
  17. Y. Liu et al., Nat. Neurosci 8, 1151–1159 (2005)CrossRefGoogle Scholar
  18. Y. Okon, L. Cakmakci, I. Nur, I. Chet, Microb. Ecol. 6, 277–280 (1980)CrossRefGoogle Scholar
  19. J. Park, T. Bansal, M. Pinelis, M.M. Maharbiz, Lab Chip 6, 611–622 (2006)CrossRefGoogle Scholar
  20. M.O. Reese, R.M. van Dam, A. Scherer, S.R. Quake, Genome Res. 13, 2348–2352 (2003)CrossRefGoogle Scholar
  21. J.P. Sullivan, A.F. Palmer, Biotechnol. Prog. 22, 1374–1387 (2006)CrossRefGoogle Scholar
  22. J.P. Sullivan, J.E. Gordon, A.F. Palmer, Biotechnol. Bioeng. 93, 306–317 (2006)CrossRefGoogle Scholar
  23. S. Takayama et al., PNAS 96, 5545–5548 (1999)CrossRefGoogle Scholar
  24. Z. Yun, Q. Lin, A.J. Giaccia, Mol. Cell. Biol. 25, 3040–3055 (2005)CrossRefGoogle Scholar
  25. R. Venkatasubramanian, M.A. Henson, N.S. Forbes, J. Theor. Biol. 242, 440–453 (2006)CrossRefMathSciNetGoogle Scholar
  26. I.D. Weiner, L.L. Hamm, Renal Physiol. 256, 957–964 (1989)Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Mike Pinelis
    • 1
  • Leonid Shamban
    • 2
  • Andreja Jovic
    • 3
  • Michel M. Maharbiz
    • 4
  1. 1.Department of Electrical Engineering and Computer ScienceUniversity of MichiganAnn ArborUSA
  2. 2.Department of Molecular, Cellular and Developmental BiologyUniversity of MichiganAnn ArborUSA
  3. 3.Department of Biomedical EngineeringUniversity of MichiganAnn ArborUSA
  4. 4.Department of Electrical Engineering and Computer ScienceUniversity of California BerkeleyBerkeleyUSA

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