Biomedical Microdevices

, 10:807

A high-yield method for generating mass-transfer gradients in elastomer microfluidics using impermeable capillaries

  • Mike Pinelis
  • Leonid Shamban
  • Andreja Jovic
  • Michel M. Maharbiz


We demonstrated a robust, inexpensive method for fabricating high-throughput gas gradient generation devices which combined classic mass-transfer limited techniques with the versatility of elastomer microfluidics. The method allowed for dozens of replicate mass-transfer gradient experiments per day, including fabrication and assembly of devices. We demonstrated how our devices can be interfaced with microfluidics and how gradient parameters can be varied. In this work, we applied the method to characterize gradients of pH and cell viability generated in mass-transfer limited cultures of HeLa cells and observed the morphology of differentiating C2C12 myoblasts in an oxygen gradient.


Oxygen Gradient Cell Culture Gas High Throughput Chemotaxis 

Supplementary material

10544_2008_9195_MOESM1_ESM.doc (5.4 mb)
Supplemental Information(DOC 5.38 MB)


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Mike Pinelis
    • 1
  • Leonid Shamban
    • 2
  • Andreja Jovic
    • 3
  • Michel M. Maharbiz
    • 4
  1. 1.Department of Electrical Engineering and Computer ScienceUniversity of MichiganAnn ArborUSA
  2. 2.Department of Molecular, Cellular and Developmental BiologyUniversity of MichiganAnn ArborUSA
  3. 3.Department of Biomedical EngineeringUniversity of MichiganAnn ArborUSA
  4. 4.Department of Electrical Engineering and Computer ScienceUniversity of California BerkeleyBerkeleyUSA

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