Biomedical Microdevices

, Volume 10, Issue 3, pp 403–410

Nano-aquarium for dynamic observation of living cells fabricated by femtosecond laser direct writing of photostructurable glass

  • Yasutaka Hanada
  • Koji Sugioka
  • Hiroyuki Kawano
  • Ikuko Shihira Ishikawa
  • Atsushi Miyawaki
  • Katsumi Midorikawa
Article

Abstract

We demonstrate the fabrication of three-dimensional (3-D) hollow microstructures embedded in photostructurable glass by a nonlinear multiphoton absorption process using a femtosecond (fs) laser. Fs laser direct writing followed by annealing and successive wet etching in dilute hydrofluoric (HF) acid solution resulted in the rapid manufacturing of microchips with 3-D hollow microstructures for the dynamic observation of living microorganisms and cells in fresh water. The embedded microchannel structure enables us to analyze the continuous motion of Euglena gracilis. A microchamber with a movable microneedle demonstrates its ability for the elucidation of the information transmission process in Pleurosira laevis. Such microchips, referred to as nano-aquariums realize the efficient and highly functional observation of microorganisms and cells.

Keywords

Nano-aquarium Microchip Microorganism Living cell Femtosecond laser Photostructurable glass 

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Yasutaka Hanada
    • 1
  • Koji Sugioka
    • 1
  • Hiroyuki Kawano
    • 2
  • Ikuko Shihira Ishikawa
    • 2
  • Atsushi Miyawaki
    • 2
  • Katsumi Midorikawa
    • 1
  1. 1.RIKEN-The Institute of Physical and Chemical ResearchSaitamaJapan
  2. 2.RIKEN-Brain Science InstituteSaitamaJapan

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