Biomedical Microdevices

, Volume 10, Issue 2, pp 233–241 | Cite as

Application of microstereolithography in the development of three-dimensional cartilage regeneration scaffolds

  • Seung-Jae Lee
  • Hyun-Wook Kang
  • Jung Kyu Park
  • Jong-Won Rhie
  • Sei Kwang Hahn
  • Dong-Woo Cho
Article

Abstract

Conventional methods for fabricating three-dimensional (3-D) tissue engineering scaffolds have substantial limitations. In this paper, we present a method for applying microstereolithography in the construction of 3-D cartilage scaffolds. The system provides the ability to fabricate scaffolds having a pre-designed internal structure, such as pore size and porosity, by stacking photopolymerized materials. To control scaffold structure, CAD/CAM technology was used to generate a scaffold pattern algorithm. Since tissue scaffolds must be constructed using a biocompatible, biodegradable material, scaffolds were synthesized using liquid photocurable TMC/TMP, followed by acrylation at the terminal ends, and photocured under UV light irradiation. The solidification properties of the TMC/TMP polymer were also assessed. To assess scaffold functionality, chondrocytes were seeded on two types of 3-D scaffold and characterized for cell adhesion. Results indicate that scaffold geometry plays a critical role in chondrocyte adhesion, ultimately affecting the tissue regeneration utility of the scaffolds. These 3-D scaffolds could eventually lead to optimally designed constructs for the regeneration of various tissues, such as cartilage and bone.

Keywords

Microstereolithography Scaffold Chondrocyte CAD/CAM 

References

  1. B. Dhariwala, E. Hunt, T. Boland, Rapid prototyping of tissue-engineering constructs using photopolymerizable hydrogels and stereolithography. Tissue Eng. 10, 1316–1322 (2004)Google Scholar
  2. D. Hutmacher, Scaffold design and fabrication technologies for engineering tissues-state of the art and future perspectives. J. Biomater. Sci. Polym. Ed. 12, 107–124 (2001)CrossRefGoogle Scholar
  3. D. Hutmacher, M. Sittinger, N.V. Risbud, Scaffold-based tissue engineering: rationale for computer-aided design and solid freeform fabrication systems. Trends Biotechnol. 22, 354–362 (2004)CrossRefGoogle Scholar
  4. I. Koji, Y. Akira, N. Fuminori, Real three-dimensional microfabrication for biodegradable polymers. Proceedings of the 26th Annual International Conference of the IEEE EMBS. (San Francisco, CA, USA, 2004), pp. 2679–2682Google Scholar
  5. I.K. Kwon, T. Matsuda, Photo-polymerized microarchitectural constructs prepared by microstereolithography (μST) using liquid acrylate-end-capped trimethylene carbonate-based prepolymers. Biomaterials. 26, 1675–1684 (2005)CrossRefGoogle Scholar
  6. I.H. Lee, D-W. Cho, An investigation on photo-polymer solidification considering laser irradiation energy in micro-stereolithography. Microsyst. Technol. 10, 592–598 (2004)CrossRefGoogle Scholar
  7. S.J. Lee, H-W. Kang, Y. Kim, G-W. Lee, G. Lim, D-W. Cho, Development of a micro-blood-typing system using micro-stereolithography. Sensor Mater. 17, 113–123 (2005)Google Scholar
  8. S.J. Lee, B. Kim, G. Lim, S.W. Kim, J.W. Rhie, D.W. Cho, Effect of three-dimensional scaffold geometry on chondrocyte adhesion. Key Eng. Mater. 342–343, 97–100 (2007)CrossRefGoogle Scholar
  9. K.F. Leong, C.M. Cheah, C.K. Chua, Solid freeform fabrication of three-dimensional scaffolds for engineering replacement tissues and organs. Biomaterials. 24, 2363–2378 (2003)CrossRefGoogle Scholar
  10. G. Mapili, Y. Lu, S. Chen, K. Roy, Laser-layered microfabrication of spatially patterned functionalized tissue-engineering scaffolds. J. Biomed. Mater. Res. Part B: Appl. Biomater. 75B, 414–424 (2005)CrossRefGoogle Scholar
  11. T. Matsuda, I.K. Kwon, S. Kidoaki, Photocurable biodegradable liquid copolymers: synthesis of acrylate-end-capped trimethylene carbonate-based prepolymers, photocuring, and hydrolysis. Biomacromolecules. 5, 295–305 (2004)CrossRefGoogle Scholar
  12. T.B.F. Woodfield, J. Malda, J. Wijn, F. Peters, J. Riesle, C.A. Blitterswijk, Design of porous scaffolds for cartilage tissue engineering using a three-dimensional fiber-deposition technique. Biomaterials. 25, 4149–4161 (2004)CrossRefGoogle Scholar
  13. S. Yang, K-F. Leong, Z. Du, C-K. Chua, The design of scaffolds for use in tissue engineering: I. Traditional factors. Tissue Eng. 7, 679–689 (2001)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Seung-Jae Lee
    • 1
  • Hyun-Wook Kang
    • 1
  • Jung Kyu Park
    • 2
  • Jong-Won Rhie
    • 3
  • Sei Kwang Hahn
    • 2
  • Dong-Woo Cho
    • 1
  1. 1.Department of Mechanical EngineeringPohang University of Science and TechnologyPohangSouth Korea
  2. 2.Department of Materials Sciences and EngineeringPohang University of Science and TechnologyPohangSouth Korea
  3. 3.Department of Plastic Surgery, College of MedicineThe Catholic University of KoreaSeoulSouth Korea

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