Biomedical Microdevices

, Volume 9, Issue 4, pp 443–449

Miniature real time PCR on chip with multi-channel fiber optical fluorescence detection module

Article

Abstract

This paper presents the design and implementation of a miniature real time PCR system consisting of a disposable reactor chip, a miniature thermal cycler, and a multi-channel fiber optical fluorescence excitation/detection module. The disposable PCR chip is fabricated by using soft photolithography by PDMS (Polydimethylsiloxane) and glass. The miniature thermal cycler has a thin film heater for heating and a fan for rapid cooling. The fiber optical detection module consists of laser, filter cube, photo-detector and 1×4 fiber optical switch. It is capable of four-well real time PCR analysis. Real-time PCR detection of E. coli stx1 has been demonstrated successfully with this system.

Keywords

Real-time PCR Lab-on-a-chip Miniaturization E. coli 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Department of Mechanical and Industrial EngineeringUniversity of TorontoTorontoCanada
  2. 2.Department of Mechanical EngineeringVanderbilt UniversityNashvilleUSA

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