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Miniature real time PCR on chip with multi-channel fiber optical fluorescence detection module

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Abstract

This paper presents the design and implementation of a miniature real time PCR system consisting of a disposable reactor chip, a miniature thermal cycler, and a multi-channel fiber optical fluorescence excitation/detection module. The disposable PCR chip is fabricated by using soft photolithography by PDMS (Polydimethylsiloxane) and glass. The miniature thermal cycler has a thin film heater for heating and a fan for rapid cooling. The fiber optical detection module consists of laser, filter cube, photo-detector and 1×4 fiber optical switch. It is capable of four-well real time PCR analysis. Real-time PCR detection of E. coli stx1 has been demonstrated successfully with this system.

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Authors and Affiliations

  1. Department of Mechanical and Industrial Engineering, University of Toronto, 5 King’s College Road, Toronto, M5S 3G8, Canada

    Q. Xiang & B. Xu

  2. Department of Mechanical Engineering, Vanderbilt University, 2301 Vanderbilt Place, Nashville, TN, 37235-1592, USA

    D. Li

Authors
  1. Q. Xiang
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  2. B. Xu
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  3. D. Li
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Correspondence to D. Li.

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Xiang, Q., Xu, B. & Li, D. Miniature real time PCR on chip with multi-channel fiber optical fluorescence detection module. Biomed Microdevices 9, 443–449 (2007). https://doi.org/10.1007/s10544-007-9048-4

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  • DOI: https://doi.org/10.1007/s10544-007-9048-4

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