Biomedical Microdevices

, Volume 9, Issue 3, pp 335–343 | Cite as

Electrokinetic measurements of dielectric properties of membrane for apoptotic HL-60 cells on chip-based device

  • Chengjun Huang
  • Ailiang Chen
  • Lei Wang
  • Min Guo
  • Jun Yu
Article

Abstract

The specific membrane capacitance and conductance of mammalian cells reflect the surface morphological complexities and barrier functions of cell membrane, respectively, and could potentially respond to cell physiological and pathological changes in a measurable manner. In this study, an electrokinetic system was developed by using negative dielectrophoretic force (nDEP force) assisted positioning and electroroation (ROT) measurement. Numerical simulations regarding the geometric model of the electrode were performed primarily for the electric field analysis. The dielectric responses of membrane for apoptotic HL-60 cells induced by bufalin were detected. The membrane capacitance of the cells was found to fall from an initial value of 15.6 ± 0.9 mF/cm2 to 6.4 ± 0.6 mF/cm2 after a 48 h treatment with 10 nM bufalin. However, the membrane conductance remained almost constant at (2.25 ± 1.1) × 103 S/m2 during the first 12 h of bufalin treatment and then increased distinctly to (4.2 ± 1.3) × 103 S/m2 thereafter. Scan electron microscopy (SEM) studies of the cells revealed a decreased complexity in cell membrane morphology following bufalin treatments, suggesting that the observed changes in the membrane capacitance was dominated by the alterations of cell surface structures. The results demonstrate that the ROT technique gives a quantitative analysis of the toxic damage by chemicals to cells and can be exploited in the testing and development of new pharmaceuticals and active cell agents.

Keywords

Cell positioning Dielectrophoretic force Electrorotation (ROT) Apoptosis Membrane capacitance Membrane conductance Bufalin 

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Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Chengjun Huang
    • 1
    • 4
  • Ailiang Chen
    • 2
    • 3
    • 4
  • Lei Wang
    • 2
    • 3
    • 4
  • Min Guo
    • 4
  • Jun Yu
    • 1
    • 4
  1. 1.Department of Electronic Science and TechnologyHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Biological Sciences and BiotechnologyTsinghua UniversityBeijingChina
  3. 3.Medical Systems Biology Research CenterTsinghua University School of MedicineBeijingChina
  4. 4.National Engineering Research Center for Beijing Biochip TechnologyBeijingChina

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