Biomedical Microdevices

, Volume 7, Issue 1, pp 47–52

In Vitro and In Vivo Characterization of MEMS Microneedles

  • Melissa Ai Ling Teo
  • Christopher Shearwood
  • Kian Chye Ng
  • Jia Lu
  • Shabbir Moochhala
Article

Abstract

Transdermal drug delivery TDD systems have many advantages but are conventionally limited by the low permeability of skin. The idea of using microneedles to painlessly penetrate the topmost impermeable stratum corneum has previously been put forward. In this paper, the fabrication of solid and hollow silicon microneedles with straight side-walls and with the following dimensions: 20–100 μm in diameter and 100–150 μm in length is described. In vitro tests demonstrate that with prior solid microneedle application, transdermal drug transport is significantly increased by 10–20 times, with the degree of enhancement being related to needle diameter. In vivo tests in diabetic animals, however, were unable to demonstrate any delivery of insulin through the hollow microneedles. It is proposed that two factors, microneedle length and tip sharpness, have to be improved for systemic drug delivery to be seen in vivo.

Key Words

transdermal drug delivery microneedles in vitro characterization diabetic rat model 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. B.W. Barry, European Journal of Pharmaceutical Sciences 14, 101–114 (2001).Google Scholar
  2. A.H. Beckett, in Dermal & Transdermal Absorption, edited by R. Brandau and B.H. Lippold (Wissenschaftliche Verlagsgesellchaft mbH, Stuttgart, 1982), p. 155.Google Scholar
  3. R.L. Bronaugh, R.F. Stewart, and E.R. Congdon, Toxicol App Pharmacol 62, 481–488 (1982).Google Scholar
  4. L. Brown and R. Langer, Annual Reviews in Medicine 39, 221–229 (1988).Google Scholar
  5. J.G.E. Gardeniers, J.W. Berenschot, M.J. deBoer, Y. Yeshurun, M. Hefetz, R. van’t Oever, and A. van den Berg, IEEE MEMS Proceedinds (Las Vegas, USA, 2002), p. 141.Google Scholar
  6. M. Goldman, Transdermal Drug Delivery: D & MD Publications 6(8), 2 (2004).Google Scholar
  7. S. Henry, D.V. McAllister, M.G. Allen, and M.R. Prausnitz, Journal of Pharmaceutical Sciences 87(8), 922–925 (1998).Google Scholar
  8. S. Kaushik, A.H Hord, D.D Denson, D.V. McAllister, S. Smitra, M.G. Allen, and M.R. Prausnitz, Anesth Analg 92(2), 502–504 (2001).Google Scholar
  9. L. Lin and A.P. Pisano, IEEE Journal of Microelectromechanical Systems 8(1), 78–84 (1999).Google Scholar
  10. W. Martanto, S. Davis, N. Holiday, J. Wang, H Gill, and M.R. Praunitz, in Proceedings of International Symposium on Controlled Release Bioactive Material (2003), vol. 666.Google Scholar
  11. J.A. Martriano, M. Cormier, J. Johnson, W.A. Young, Buttery M.K. Nyam, and P.E. Daddona, Pharm Res 19(1), 63–70 (2002).Google Scholar
  12. D.V. McAllister, O.M. Wang, S.P. Davis, J.K. Park, P.J. Canatella, M.G. Allen, and M.R. Prausnitz, Proceedings of the National Academy of Science 100(24), 13755–13760 (2003).Google Scholar
  13. J.A. Mikszta, J.B. Alarcon, J.M Brittingham, D.E. Sutter, R.J. Pettis, and N.G. Harvey, Nature Medicine 8(4), 415–419 (2002).Google Scholar
  14. L.S. Morris and R.M. Schulz, J. Clin. Pharm. Ther. 17, 283–295 (1992).Google Scholar
  15. I. Papautsky and J. Brazzle, IEEE Transactions on Biomedical Engineering 47(6), 812–819 (2000).Google Scholar
  16. V.P. Shah, in Transdermal Drug Delivery 2nd edn., edited by R.H. Guy and J. Hadgraft (Marcel Dekker Inc, New York, 2003), p. 365.Google Scholar
  17. B. Stoeber and D. Liepmann, in 1st Annual International Conference on Microtechnologies (Lyon, France, 2000) poster 34.Google Scholar
  18. World Health Organization, Chronicle 39(2), 51–56 (1985).Google Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Melissa Ai Ling Teo
    • 1
  • Christopher Shearwood
    • 2
  • Kian Chye Ng
    • 1
  • Jia Lu
    • 1
  • Shabbir Moochhala
    • 1
  1. 1.Defence Science OrganisationDefence Medical & Environmental Research InstituteSingapore
  2. 2.School of Mechanical & Production EngineeringSingapore

Personalised recommendations