Biochemistry (Moscow)

, 70:1321

Effect of Enzyme Preparation from the Marine Mollusk Littorina kurila on Fucoidan from the Brown Alga Fucus distichus

  • M. I. Bilan
  • M. I. Kusaykin
  • A. A. Grachev
  • E. A. Tsvetkova
  • T. N. Zvyagintseva
  • N. E. Nifantiev
  • A. I. Usov
Article

DOI: 10.1007/s10541-005-0264-3

Cite this article as:
Bilan, M.I., Kusaykin, M.I., Grachev, A.A. et al. Biochemistry (Moscow) (2005) 70: 1321. doi:10.1007/s10541-005-0264-3

Abstract

A fucoidanase preparation from the marine mollusk Littorina kurila cleaved some glycosidic bonds in fucoidan from the brown alga Fucus distichus, but neither fucose nor lower oligosaccharides were produced. The main product isolated from the incubation mixture was a polysaccharide built up of disaccharide repeating units →3)-α-L-Fucp-(2,4-di-SO3)-(1→4)-α-L-Fucp-(2SO3)-(1→, the structure coinciding with the idealized formula proposed for the initial substance. A polymer fraction with the same carbohydrate chain but sulfated only at positions 2 and nonstoichiometrically acetylated at positions 3 and 4 of fucose residues was isolated as a minor component. It is suggested that the native polysaccharide should contain small amounts of non-sulfated and non-acetylated fucose residues, and only their glycosidic bonds are cleaved by the enzyme. The enzymatic hydrolysis showed that irregular regions of the native polysaccharide containing acetylated and partially sulfated repeating units were assembled in blocks.

Key words

brown algae fucoidan Fucus distichus fucoidanase Littorina kurila marine mollusks 

Copyright information

© MAIK "Nauka/Interperiodica" 2005

Authors and Affiliations

  • M. I. Bilan
    • 1
  • M. I. Kusaykin
    • 2
  • A. A. Grachev
    • 1
  • E. A. Tsvetkova
    • 1
  • T. N. Zvyagintseva
    • 2
  • N. E. Nifantiev
    • 1
  • A. I. Usov
    • 1
  1. 1.Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Pacific Institute of Bioorganic Chemistry, Far-Eastern BranchRussian Academy of SciencesVladivostokRussia

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