Biochemistry (Moscow)

, Volume 70, Issue 5, pp 576–583

Methylation of the Tumor Suppressor Gene RASSF1A in Human Tumors

Article

DOI: 10.1007/s10541-005-0151-y

Cite this article as:
Pfeifer, G.P. & Dammann, R. Biochemistry (Moscow) (2005) 70: 576. doi:10.1007/s10541-005-0151-y

Abstract

Loss of heterozygosity of a segment at 3p21.3 is frequently observed in lung cancer and several other carcinomas. We have identified the Ras-association domain family 1A gene (RASSF1A), which is localized at 3p21.3 in a minimum deletion sequence. De novo methylation of the RASSF1A promoter is one of the most frequent epigenetic inactivation events detected in human cancer and leads to silencing of RASSF1A expression. Hypermethylation of RASSF1A was frequently found in most major types of human tumors including lung, breast, prostate, pancreas, kidney, liver, cervical, thyroid and many other cancers. The detection of RASSF1A methylation in body fluids such as serum, urine, and sputum promises to be a useful marker for early cancer detection. The functional analysis of RASSF1A reveals a potential involvement of this protein in apoptotic signaling, microtubule stabilization, and cell cycle progression.

Key words

RASSF1A DNA methylation tumor suppressor cancer 

Abbreviations

RASSF1

Ras association domain family 1

NORE1

novel Ras effector 1

LOH

loss of heterozygosity

RA domain

RalGDS/AF6 Ras-association domain

aa

amino acid

HPV

human papilloma virus

ATM

ataxia telangiectasia mutated

C1

protein kinase C conserved region 1

SCLC

small cell lung cancer

NSCLC

non-small cell lung cancer

Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  1. 1.Department of Biology, Beckman Research InstituteCity of Hope Cancer CenterDuarteUSA
  2. 2.AG Tumorgenetik der Medizinischen FakultatMartin-Luther Universitat Halle-WittenbergHalle/SaaleGermany

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