Response of Arabidopsis thaliana root growth to phosphorus and its relation to media chemical composition
The interaction between phosphorus (P) and other media components alters root development and masks the plant response and thus limits the ability to correctly identify P-deficiency response (pdr) mutants. This study aims to assess changes in root development caused by different composition of growth media normally used in Arabidopsis research and to study their effects on pdr-mutant screening. Primary root growth of four genotypes was analyzed in media differing in P concentrations: half-strength Murashige and Skoog (½ MS) and Somerville and Ogren (SO). The effects of nitrogen source and Fe on root growth were investigated in each medium separately and in a mixture. We found that the primary root length of all genotypes grown on ½ MS was reduced in comparison with plants grown on SO medium. The mutant pdr9 was the most sensitive in ½ MS, This mutant was also hypersensitive to Fe that intensified its sensitivity to ammonium. Ammonium increased the root inhibition caused by Fe also in wild-type plants. In conclusion, on the basis of our study we recommend to use SO medium, which ensures an efficient selection to screen for pdr mutants through root growth. Moreover, nitrogen sources in the media other than nitrate should be taken carefully.
Additional key wordsammonium sensitivity iron hypersensitivity nutrient interactions phosphate deficiency quiescent center identity
Murashige and Skoog
phosphate deficiency response1
phosphate starvation response 1
Somerville and Ogren
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- Delatorre, C.A.: Phosphate Deficiency Response: Searching for the Signaling Pathway. — Lambert Academic Publishing, Köln, Germany 2009.Google Scholar
- González-Mendoza, V., Zurita-Silva, A., Sánchez-Calderón, L., Sánchez-Sandoval, M.E., Oropeza-Aburto, A., Gutiérrez, A.D., Alatorre-Cobos, F., Herrera-Estrella, L.: Apsr1, a novel gene required for meristem maintenance, is negatively regulated by low phosphate availability. — Plant Sci. 205–206: 2–12, 2013.CrossRefPubMedGoogle Scholar
- Jain, A., Poling, M.D., Smith, A.P., Nagarajan, V.K., Lahner, B., Meaghe,r R.B., Raghothama, K.G.: Variations in the composition of gelling agents affect morphophysiological and molecular responses to deficiencies of phosphate and other nutrients. — Plant Physiol. 150: 1033–1049, 2009.CrossRefPubMedPubMedCentralGoogle Scholar
- Somerville, C.R., Ogren, W.: Isolation of photorespiration mutants in Arabidopsis thaliana. -In: Edelman, M. (ed.): Methods in Chloroplast Biology. Pp. 129–138. Elsevier Biomedical Press, Amsterdam 1982.Google Scholar
- Stefanovic, A. Ribot, C., Rouached, H., Wang, Y., Chong, J., Belbahri, L., Delessert, S., Poirie, Y: Members of the pho1 gene family show limited functional redundancy in phosphate transfer to the shoot, and are regulated by phosphate deficiency via distinct pathways. - Plant J. 50: 982–994, 2007.CrossRefPubMedGoogle Scholar