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Biologia Plantarum

, Volume 61, Issue 1, pp 115–126 | Cite as

Cucumber PDR8/ABCG36 and PDR12/ABCG40 plasma membrane proteins and their up-regulation under abiotic stresses

  • M. MigockaEmail author
  • A. Papierniak
  • A. Rajsz
Original Paper

Abstract

The cucumber genes CsPDR8/CsABCG36 and CsPDR12/CsABCG40 encode two similar pleiotropic drug resistance proteins (ABCGPDRs) belonging to the large ABC family of multispecific ATP-dependent transporters. We have already shown that the amount of root CsPDR8/CsABCG36 and CsPDR12/CsABCG40 transcripts is markedly elevated by phytohormones related to the plant response to environmental constraints, suggesting the involvement of both genes in hormone-mediated reactions to stresses. To further characterize the function and regulation of CsPDR8/CsABCG36 and CsPDR12/CsABCG40, we determined the subcellular localization of the predicted CsPDR8/CsABCG36 and CsPDR12/CsABCG40 proteins in cucumber and performed a transcriptional analysis of genes encoding these proteins under different abiotic stresses (heavy metals, salinity, osmotic stress, and oxidative stress) and redox perturbations. In addition, the activities of antioxidative enzymes as well as the content of hydrogen peroxide and superoxide were measured in cucumber roots to monitor the redox perturbations under all experimental conditions. Western blot analysis of membrane fractions prepared from cucumber roots with specific antibodies raised against the peptides corresponding to sequences unique to CsPDR8/CsABCG36 and CsPDR12/CsABCG40 revealed that both proteins localize to the plasma membrane. The transcript abundance and the plasma membrane protein content closely correlated with the stress severity and the hydrogen peroxide content but not with the superoxide anion content. Based on the results obtained so far, we may conclude that CsPDR8/CsABCG36 and CsPDR12/CsABCG40 are up-regulated under multiple stress conditions and redox perturbations and that the H2O2 and stress-related phytohormones can act as signaling molecules affecting the expression of both cucumber genes.

Additional key words

gene expression heavy metals osmotic stress oxidative stress reactive oxygen species salt stress 

Abbreviations

ABA

abscisic acid

ABC

ATP binding cassette

ABCGPDR

pleiotropic drug resistance proteins of ABC subfamily G

ACC

1-amino-cyclopropane-1-carboxylic acid

APX

ascorbate peroxidase

ASA

ascorbic acid

CAT

catalase

2,4-D

2,4-dichlorophenoxyacetic acid

DEX

dextran

DTT

dithiothreitol

GR

glutathione reductase

GR

glutathione reductase

GSH

reduced glutathione

GSSG

glutathione disulphide

JA

jasmonic acid

MES

2-(N-morpholino)ethanesulfonic acid

PEG

polyethylene glycol

PMSF

phenylmethylsulfonyl fluoride

ROS

reactive oxygen species

SA

salicylic acid

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Supplementary material

10535_2016_679_MOESM1_ESM.pdf (133 kb)
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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Department of Plant Molecular Physiology, Institute of Experimental BiologyWrocław UniversityWrocławPoland

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