Biologia Plantarum

, Volume 58, Issue 4, pp 768–772 | Cite as

Cell membrane integrity, callose accumulation, and root growth in aluminum-stressed sorghum seedlings

  • E. J. Too
  • A. S. Carlsson
  • A. O. Onkware
  • B. A. Were
  • M. Geleta
  • T. Bryngelsson
  • S. Gudu
Brief Communication

Abstract

Aluminum stress usually reduces plant root growth due to the accumulation of Al in specific zones of the root apex. The objectives of this study were to determine the localization of Al in the root apex of Sorghum bicolor (L.) Moech. and its effects on membrane integrity, callose accumulation, and root growth in selected cultivars. Seedlings were grown in a nutrient solution containing 0, 27, or 39 μM Al3+ for 24, 48, and 120 h. The Al stress significantly reduced root growth, especially after 48 and 120 h of exposure. A higher Al accumulation, determined by fluorescence microscopy after staining with a Morin dye, occurred in the root extension zone of the sensitive cultivar than in the tolerant cultivar. The membrane damage and callose accumulation were also higher in the sensitive than resistant cultivar. It was concluded that the Al stress significantly reduced root growth through the accumulation of Al in the root extension zone, callose accumulation, and impairment of plasma membrane integrity.

Additional key words

aluminum tolerance root extension zone 

Abbreviations

BIO-EARN

Eastern African Regional Programme and Research Network for Biotechnology, Biosafety and Biotechnology Policy Development

ICRISAT

International Crops Research Institute for the Semi-Arid Tropics

SIDA

Swedish International Development Agency

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • E. J. Too
    • 1
  • A. S. Carlsson
    • 2
  • A. O. Onkware
    • 1
  • B. A. Were
    • 1
  • M. Geleta
    • 2
  • T. Bryngelsson
    • 2
  • S. Gudu
    • 3
  1. 1.Department of Biological SciencesUniversity of EldoretEldoretKenya
  2. 2.Department of Plant BreedingSwedish University of Agricultural SciencesAlnarpSweden
  3. 3.Rongo University CollegeConstituent College of Moi UniversityRongoKenya

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