Biologia Plantarum

, Volume 58, Issue 4, pp 681–688 | Cite as

The genetic basis of durum wheat germination and seedling growth under osmotic stress

  • M. Nagel
  • S. Navakode
  • V. Scheibal
  • M. Baum
  • M. Nachit
  • M. S. Röder
  • A. Börner
Original Papers

Abstract

Durum wheat (Triticum turgidum L. var. durum) is mainly produced under rainfed but often sub-optimal moisture conditions in the Mediterranean basin. A set of 114 durum wheat recombinant inbred lines (RILs) developed from the cross of cultivars Omrabi5 × Belikh2 were tested for the ability to tolerate moisture deficiency at the germination and early seedling growth stage. The stress was imposed by exposing the germinating grain to 12 % polyethylene glycol. It induced a measurable reduction in root length, shoot length, and the percentage of normal seedlings. The germination and seedling growth of Belikh2 were more strongly inhibited than those of Omrabi5, and both parents were outperformed by > 50 % of the RILs. A quantitative trait locus (QTL) analysis was carried out by first assembling a linkage map from 265 informative microsatellites. Composite interval mapping revealed nine QTL spread over seven chromosomes. Five of these were associated with coleoptile length, and one of the five explained nearly 29 % of the relevant phenotypic variance. The coleoptile length was significantly correlated with the seedling growth, plant height, and thousand kernel mass derived from field-grown plants of the same RIL population.

Additional key words

drought stress polyethylene glycol QTL recombinant inbred lines seed size seed vigour Triticum durum 

Abbreviations

BARC

Beltsville Agricultural Research Centre

GWM

Gatersleben Wheat Microsatellite

H2

broad-sense heritability

ICARDA

International Center for Agricultural Research in the Dry Areas

ISTA

International Seed Testing Association

LOD

logarithm of odds

NS

normal seedling

PEG

polyethylene glycol

QTL

quantitative trait locus

RIL

recombinant inbred line

TG

total germinated seedlings

TI

tolerance index

WMC

Wheat Microsatellite Consortium

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • M. Nagel
    • 1
  • S. Navakode
    • 1
  • V. Scheibal
    • 1
  • M. Baum
    • 2
  • M. Nachit
    • 2
  • M. S. Röder
    • 1
  • A. Börner
    • 1
  1. 1.Leibniz Institute of Plant Genetics and Crop Plant Research (IPK Gatersleben)Stadt SeelandGermany
  2. 2.International Center for Agricultural Research in the Dry Areas (ICARDA)AleppoSyria

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