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Biologia Plantarum

, Volume 58, Issue 2, pp 283–295 | Cite as

Candidate gene expression profiling in two contrasting tomato cultivars under chilling stress

  • A. CaffagniEmail author
  • N. Pecchioni
  • E. Francia
  • D. Pagani
  • J. Milc
Original Papers

Abstract

Tomato (Solanum lycopersicum Mill.) is sensitive to chilling stress during all stages of plant development. Genetic variation for chilling tolerance exists between cultivated tomato and its related wild species, but intra-specific variation has not been thoroughly investigated so far. Seedlings of 63 tomato accessions were evaluated under low temperature and two contrasting cultivars were identified for the trait: Albenga and San Marzano, the former being more chillingtolerant. To clarify the molecular mechanisms of chilling tolerance in tomato, changes in candidate gene expressions in the two tomato genotypes were analysed, using quantitative RT-PCR. Candidate genes were chosen among those known to be induced by chilling and/or with putative roles in CBF/DREB and ROS-mediated pathways. Results show that besides a CBF regulon, whose function is conserved, ROS and C2H2-type zinc finger protein-mediated cold signalling pathways were also involved in chilling tolerance. Under the chilling stress, the up-regulation of respective transcripts was consistently higher in the chilling-tolerant genotype than in the chilling-sensitive ones.

Additional key words

C2H2-type zinc finger protein CBF/DREB pathway ROS-mediated pathway Solanum lycopersicum 

Abbreviations

ABA

abscisic acid

AP2/ERF

APETALA 2/ethylene response factor

Aux/IAA

auxin/indole-3-acetic acid

bZIP

basic region-leucine zipper

CBF/DREB

C-repeat binding factor/dehydration-responsive element binding

COR

cold-regulated

CRT/DRE

C-repeat/dehydration responsive element

Ct

cycle threshold

DHNs

dehydrins

DMRT

Duncan’s multiple range test

EAR-motif

ERF-associated amphiphilic repression motif

EF-1-α

elongation factor 1-α

EL

electrolyte leakage

ERF

ethylene response factor

FAD

fatty acid desaturase

GLM

generalized linear model

ILs

introgression lines

LEA

late embryogenesis abundant

MYB

myeloblastosis

NLS

nuclear localization signal

NTC

no template control

RT-qPCR

reverse transcription-quantitative PCR

RCBD

randomized complete block design

REST©

relative expression software tool©

ROS

reactive oxygen species

SGN

sol genomics network

TFs

transcription factors

USP

universal stress protein

WT

wild type

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Supplementary material

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • A. Caffagni
    • 1
    Email author
  • N. Pecchioni
    • 1
    • 2
  • E. Francia
    • 1
    • 2
  • D. Pagani
    • 3
  • J. Milc
    • 1
    • 2
  1. 1.Interdepartmental Research Centre BIOGEST-SITEIAUniversity of Modena and Reggio EmiliaReggio EmiliaItaly
  2. 2.Department of Life SciencesUniversity of Modena and Reggio EmiliaReggio EmiliaItaly
  3. 3.Genomics Research CentreFiorenzuola d’ArdaItaly

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