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Biologia Plantarum

, Volume 57, Issue 4, pp 675–683 | Cite as

Peroxiredoxins are involved in two independent signalling pathways in the abiotic stress protection in Vitis vinifera

  • P. Vidigal
  • R. Carvalho
  • S. Amâncio
  • L. Carvalho
Original Papers

Abstract

Peroxiredoxins (Prxs) play major roles in preventing oxidative damage and their function is consistent with the presence of Prx isoforms in most, if not all, cellular compartments and their expression is dependent on environmental conditions. The aim of this study was to identify and characterize genes encoding Prxs in Vitis vinifera. Quantitative real time polymerase chain reaction (qRT PCR) was used to determine their response to irradiance, heat, and water stress. We identified seven vvprx genes, two of which were especially responsive to water stress, followed by heat stress, but no major changes were observed after high irradiance. The vvprxIIF targeted to mitochondria was the most responsive to water stress and it might be involved in drought tolerance through H2O2 signalling. The vvprxII-2, a putative PrxII, is targeted to the chloroplasts and was the most responsive to heat stress. It might be related with abscisic acid-dependent thermotolerance. Additional key words: abscisic acid, antioxidants, grapevine, heat stress, photosynthesis, qRT PCR, water stress.

Keywords

Water Stress Heat Stress High Irradiance Vitis Vinifera Peroxide Detoxification 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

ABA

abscisic acid

APX

ascorbate peroxidase

CAT

catalase

cys

cystein

DHAR

dihydroascorbate reductase

Fv/Fm

variable to maximum chlorophyll fluorescence ratio

GOR

glutathione reductase

PPDF

photosynthetic photon flux density

Prx

peroxiredoxin

qRT-PCR

quantitative real time polymerase chain reaction

ROS

reactive oxygen species

SOD

superoxide dismutase

WS

water stress

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • P. Vidigal
    • 1
  • R. Carvalho
    • 1
  • S. Amâncio
    • 1
  • L. Carvalho
    • 1
  1. 1.DRAT/CBAA, Instituto Superior de AgronomiaUniversidade Técnica de LisboaLisboaPortugal

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