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Biologia Plantarum

, Volume 57, Issue 3, pp 531–539 | Cite as

Impact of phloem girdling on leaf gas exchange and hydraulic conductance in hybrid aspen

  • A. SellinEmail author
  • A. Niglas
  • E. Õunapuu
  • A. Karusion
Article

Abstract

We investigated phloem-xylem interactions in relation to leaf hydraulic capacity in hybrid aspen (Populus tremula L. × P. tremuloides Michx.) by using phloem girdling method. Removal of bark tissues (phloem girdling) at the branch base resulted in a substantial decline in stomatal conductance (gS), net photosynthetic rate (PN), and leaf hydraulic efficiency, and in increase of leaf water potential (ΨL). Although gS declined more than PN (83 versus 78 %), the ratio of intercellular to ambient CO2 concentrations (ci/ca) increased from 0.67 to 0.87 in three days after girdling. Girdling induced a decrease in leaf hydraulic conductance (KL) on average by 43 % (P = 0.006). The changes in gS and leaf conductance to water vapour were co-ordinated with KL only in girdled branches whereas intrinsic water-use efficiency was invariant to KL. The declines in KL with girdling were not accompanied by changes in potassium ion concentration ([K+]), electrical conductivity, or pH of xylem sap. The results suggest that phloem girdling at the branch base does not influence the recirculation of ions between the phloem and xylem in hybrid aspen and the decrease of KL in response to the manipulation is not related to changes in [K+] and total ionic content of xylem sap.

Additional key words

leaf water potential net photosynthetic rate phloem-xylem interactions potassium stomatal conductance water-use efficiency xylem sap 

Abbreviations

ci/ca

ratio of intercellular to ambient CO2 concentration

E

transpiration rate

gL

leaf conductance to water vapour

gS

stomatal conductance to water vapour

IWUE

intrinsic water-use efficiency

[K+]

potassium ion concentration of xylem sap

KL

leaf hydraulic conductance

PN

net photosynthetic rate

TL

leaf temperature

σsap

electrical conductivity of xylem sap

ΨB

branch xylem water potential

ΨL

leaf water potential

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • A. Sellin
    • 1
    Email author
  • A. Niglas
    • 1
  • E. Õunapuu
    • 1
  • A. Karusion
    • 1
  1. 1.Institute of Ecology and Earth SciencesUniversity of TartuTartuEstonia

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