Biologia Plantarum

, Volume 57, Issue 3, pp 442–448 | Cite as

Characterization and gene mapping of a chlorophyll-deficient mutant clm1 of Triticum monococcum L.

  • M. J. Ansari
  • A. Al-Ghamdi
  • R. Kumar
  • S. Usmani
  • Y. Al-Attal
  • A. Nuru
  • A. A. Mohamed
  • K. Singh
  • H. S. Dhaliwal
Article
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Abstract

Diploid wheat Triticum monococcum L. is a model plant for wheat functional genomics. Chlorophyll-deficient mutant (clm1) was identified during manual screening of the ethyl methanesulphonate (EMS)-treated M2 progenies of T. monococcum accession pau14087 in the field. The clm1 mutant, due to significantly decreased chlorophyll content compared with the wild-type (WT), exhibited pale yellow leaves which slowly recovered to green before flowering. The clm1 mutant showed early flowering, reduced number of tillers, trichome length and density, and different shape as compared with the WT. At the same time, clm1 mutant culm had more chlorophyll-containing parenchymatous tissues compared to WT, presumably to absorb more sunlight for photosynthesis. Genetic analysis indicated that the clm1 mutation was monogenic recessive. The clm1 mutant was mapped between Xgwm473 and Xwmc96 SSR markers, with genetic distances of 2.1 and 2.6 cM, respectively, on the 7AmL chromosome.

Additional key words

bulk segregant analysis diploid wheat ethylmethane sulfonate gene mapping SSR marker 

Abbreviations

BSA

bulk segregant analysis

Chl

chlorophyll

clm1

chlorophyll-deficient mutant

EMS

ethylmethane sulfonate

M2

second generation after mutagenesis

PCR

polymerase chain reaction

RILs

recombinant inbred lines

SEM

scanning electron microscopy

T.S.

transverse section

WT

wild-type

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • M. J. Ansari
    • 1
    • 6
  • A. Al-Ghamdi
    • 1
  • R. Kumar
    • 2
  • S. Usmani
    • 3
  • Y. Al-Attal
    • 1
  • A. Nuru
    • 1
  • A. A. Mohamed
    • 1
  • K. Singh
    • 4
  • H. S. Dhaliwal
    • 5
  1. 1.Department of Plant Protection, College of Food and Agriculture SciencesKing Saud UniversityRiyadhKSA
  2. 2.Department of BiotechnologyICFAI UniversityDehradunIndia
  3. 3.D.K.M. College for WomenThiruvalluvar UniversityVelloreIndia
  4. 4.School of Agricultural BiotechnologyPunjab Agricultural UniversityLudhianaIndia
  5. 5.Akal School of BiotechnologyEternal UniversityBaru SahibIndia
  6. 6.Department of BiotechnologyIndian Institute of TechnologyRoorkeeIndia

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