Biologia Plantarum

, Volume 57, Issue 2, pp 245–254 | Cite as

Differential expression of VRN1 and other MADS-box genes in Festuca pratensis selections with different vernalization requirements

  • Å. Ergon
  • H. Hamland
  • O. A. Rognli
Original Papers


Most perennial and winter annual temperate grasses have a vernalization requirement (VR) for flowering, that is, they require a cold period before they can flower in response to long days. From a F1 mapping population of the outbreeding perennial forage grass Festuca pratensis Huds. (meadow fescue) previously used to map several quantitative trait loci (QTLs) for VR, we produced two F2 populations divergently selected for high or low VR. The two populations were characterised for flowering behaviour and gene expression of VRN1 as well as other MADS-box genes with a putative function in the induction of flowering. Expression of FpVRN1 and the VRN1-like genes FpMADS2 and FpMADS3 was associated with flowering but the response of gene expression to vernalization differed between genes and populations. The expression of the SVP-like genes FpMADS10 and FpMADS16 was not affected by vernalization and did not differ between the two F2 populations.

Additional key words

FUL induction of flowering MADS2 MADS3 MADS10 MADS16 meadow fescue SVP transition to reproductive development 



days to heading






polymerase chain reaction


proportion of heading shoots


quantitative trait loci


reverse transcription


single nucleotide polymorphism




vernalization requirement


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Dept. of Plant and Environmental SciencesNorwegian University of Life SciencesÅsNorway
  2. 2.Dept. of Medical Biology, Faculty of Health SciencesUniversity of TromsøTromsøNorway
  3. 3.HamarNorway

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