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Biologia Plantarum

, Volume 57, Issue 2, pp 313–324 | Cite as

Differential ozone sensitivity interferes with cadmium stress in poplar clones

  • A. CastagnaEmail author
  • D. Di Baccio
  • R. TognettiEmail author
  • A. Ranieri
  • L. Sebastiani
Original Papers

Abstract

Information on plant responses to combined ozone and cadmium stresses are scarce and limited to herbaceous species. In this research, two poplar clones (I-214 and Eridano), differently sensitive to O3, were grown for 5 weeks in pots supplied with 0, 53.5, and 160.5 mg(Cd) kg−1 (soil d.m.) and then exposed to 15-d O3 fumigation (0.06 mm3 dm−3, 5 h a day). The effects of the two stressors, alone or in combination, on Cd, Ca, Fe, and Zn accumulation in above-nad below-ground organs, photosynthesis, leaf pigments, and accumulation of H2O2 and NO were investigated. Cadmium induced a reduction in stomatal conductance and a significant accumulation of H2O2 and NO in both clones nad negatively affected the carotenoid content in I-214. Ozone, on the other hand, counteracted Cd accumulation in the above-ground organs and significantly increased the xanthophyll de-epoxidation state indicating photoinhibition in O3-treated plants. Surprisingly, O3 alone or in combination with Cd decreased H2O2 accumulation in I-214. The NO production was generally stimulated by Cd, whereas it decreased following O3 exposure in I-214. The overall data indicate that Cd and O3 induced clone specific responses. Moreover, when they were applied in combination, antagonistic rather than synergistic effects were observed.

Additional key words

carotenoids hydrogen peroxide nitric oxide net photosynthetic rate Populus spp. stomatal conductance xanthophyll cycle 

Abbreviations

A

anteraxanthin

PNmax

maximum photosynthetic rate

Chl

chlorophyll

ci

internal CO2 concentration

cPTIO

2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide

DAF-FM DA

amino-5-methylamino-2′,7′-difluorescein diacetate

DEPS

de-epoxidation index

gs

stomatal conductance

PPFD

photosynthetic photon flux density

ROS

reactive oxygen species

SNP

sodium nitroprusside

V

violaxanthin

VPD

vapour pressure deficit

Z

zeaxanthin

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© Springer Science+Business Media B.V. 2013

Authors and Affiliations

  1. 1.Dipartimento di Biologia delle Piante AgrarieUniversità di PisaPisaItaly
  2. 2.BioLabs, ISVScuola Superiore Sant’AnnaPisaItaly
  3. 3.Dipartimento di Bioscienze e TerritorioUniversità degli Studi del MolisePescheItaly

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