Biologia Plantarum

, Volume 57, Issue 1, pp 164–168 | Cite as

Thidiazuron and silver nitrate enhanced gynogenesis of unfertilized ovule cultures of Cucumis sativus

  • J. W. Li
  • S. W. Si
  • J. Y. Cheng
  • J. X. Li
  • J. Q. Liu
Brief Communication
First Online:
Received:
Accepted:

Abstract

Gynogenesis of Chinese long cucumber (Cucumis sativus L.) was obtained from unpollinated ovules cultured on cucumber basal medium (CBM) supplemented with thidiazuron (TDZ) and in some experiments AgNO3. High induction frequencies (7.85–12.14 %) were induced from unpollinated ovules at the time of anthesis at 0.03–0.07 mg dm−3 TDZ. Histological analysis indicated that embryo sacs developed completely at the time of anthesis. Further, the highest plant regeneration rate was achieved at CBM supplemented with 0.05 mg dm−3 a-naphthaleneacetic acid, 0.2 mg dm−3 6-benzyladenine and 5–10 mg dm−3 AgNO3. Flow cytometry analysis showed that 80 % of the regenerated plants were haploid. Histological micrographs and ploidy level analyses clearly revealed initiation, development, and germination of embryos from the unpollinated ovules.

Additional key words

auxin cucumber cytokinin embryogenesis female gametophytes haploid 

Abbreviations

BA

6-benzyladenine

CBM

cucumber basal medium

DBA

days before anthesis

DH

doubled haploid

NAA

α-naphthaleneacetic acid

TDZ

thidiazuron

This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Agarwal, P.K., Agarwal, P., Custers, J.B.M., Liu, C.M., Bhojwani, S.S.: PCIB an antiauxin enhances microspore embryogenesis in microspore culture of Brassica juncea. — Plant Cell Tissue Organ Cult. 86: 201–210, 2006.CrossRefGoogle Scholar
  2. Aleza, P., Juárez, J., Cuenca, J., Ollitrault, P., Navarro, L.: Recovery of citrus triploid hybrids by embryo rescue and flow cytometry from 2x × 2x sexual hybridisation and its application to extensive breeding programs. — Plant Cell Rep. 29: 1023–1034, 2010.PubMedCrossRefGoogle Scholar
  3. Diao, W.P., Jia, Y.Y., Song, H., Zhang, X.Q., Lou, Q.F., Chen, J.F.: Efficient embryo induction in cucumber ovary culture and homozygous identification of the regenetants using SSR markers. — Sci. Hort. 119: 246–251, 2009.CrossRefGoogle Scholar
  4. Elviana, M., Rohani, E.R., Ismanbizan, I., Normah, M.N.: Morphological and histological changes during the somatic embryogenesis of mangosteen. — Biol. Plant. 55: 731–736, 2011.CrossRefGoogle Scholar
  5. Gémes-Juhász, A., Balogh, P., Ferenczy, A., Kristóf, Z.: Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber (Cucumis sativus L.). — Plant Cell Rep. 21: 105–111, 2002.CrossRefGoogle Scholar
  6. Kumar, H.G.A., Murthy, H.N., Paek, K.Y.: Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L. — Sci. Hort. 98: 213–222, 2003.CrossRefGoogle Scholar
  7. Kumar, V., Ramakrishna, A., Ravishankar, G.A.: Influence of different ethylene inhibitors on somatic embryogenesis and secondary embryogenesis from Coffea canephora P ex Fr. — In Vitro cell. dev. Biol. Plant 43: 602–607, 2007.CrossRefGoogle Scholar
  8. Leroux, B., Carmoy, N., Giraudet, D., Potin, P., Larher, F., Bodin, M.: Inhibition of ethylene biosynthesis enhances embryogenesis of cultured microspores of Brassica napus. — Plant Biotechnol. Rep. 3: 347–353, 2009.CrossRefGoogle Scholar
  9. Ma, G.H., He, C.X., Ren H., Zhang, Q.M., Li, S.J., Zhang, X.H., Eric, B.: Direct somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum. — Biol. Plant. 54: 361–365, 2010.CrossRefGoogle Scholar
  10. Palmer, C., Keller, W.A., Kasha, K.J.: Haploids in Crop Improvement. Vol. II. — Springer, Berlin — Heidelberg 2005.CrossRefGoogle Scholar
  11. Park, S.Y., Moon, H.K., Murthy, H.N., Kim, Y.W.: Improved growth and acclimatization of somatic embryo-derived Oplopanax elatus plantlets by ventilated photoautotrophic culture. — Biol. Plant. 55: 559–562, 2011.CrossRefGoogle Scholar
  12. Sauer, U., Wilhelm E.: Somatic embryogenesis from ovaries, developing ovules and immature zygotic embryos, and improved embryo development of Castanea sativa. — Biol. Plant. 49: 1–6, 2005.CrossRefGoogle Scholar
  13. Shalaby, T.A.: Factors affecting haploid induction through in vitro gynogenesis in summer squash (Cucurbita pepo L.). — Sci. Hort. 115: 1–5, 2007.CrossRefGoogle Scholar
  14. Sharma, S.K., Millam, S.: Somatic embryogenesis in Solanum tuberosum L.: a histological examination of key developmental stages. — Plant Cell Rep. 23: 115–119, 2004.PubMedGoogle Scholar
  15. Song, H., Lou, Q.F., Luo, X.D., Wolukau, J.N., Diao, W.P., Qian, C.T., Chen, J.F.: Regeneration of doubled haploid plants by androgenesis of cucumber (Cucumis sativus L.). — Plant Cell Tissue Organ Cult. 90: 245–254, 2007.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • J. W. Li
    • 1
  • S. W. Si
    • 1
  • J. Y. Cheng
    • 1
  • J. X. Li
    • 2
  • J. Q. Liu
    • 1
  1. 1.Open Key Laboratory of Horticultural Plant Physiology and Genetic Improvement, College of Horticultural SciencesHenan Agricultural UniversityZhengzhouHenan, P.R. China
  2. 2.Zhengzhou Vegetable Crops Research InstituteZhengzhouHenan, P.R. China

Personalised recommendations