Biologia Plantarum

, Volume 57, Issue 2, pp 281–290 | Cite as

Wheat truncated hemoglobin interacts with photosystem I PSK-I subunit and photosystem II subunit PsbS1

  • D. Y. Kim
  • M. J. Hong
  • Y. J. Lee
  • M. B. Lee
  • Y. W. Seo
Original Papers

Abstract

Recently, the truncated hemoglobin gene (trHb) was discovered in plant species, however, its role has not yet been determined. In this study, the gene expression of wheat trHb (TatrHb) was analyzed under various biotic and abiotic stresses. TatrHb transcript levels increased in NaCl-treated leaves and gibberellic acid (GA3)-treated roots. In addition, sodium nitroprusside (SNP), a nitric oxide donor, induced an increase in TatrHb transcript levels in roots and leaves. A yeast two-hybrid assay (YIIH) was used to screen a hypoxia-treated wheat seedling library with the goal of determining the putative function of TatrHb. In this YIIH assay, photosynthesis-related genes that showed high homology to the Hordeum vulgare chloroplast photosystem I PSK-I subunit and Zea mays photosystem II subunit PsbS1 were detected and their interactions with TatrHb were confirmed. Subcellular localization of a TatrHb-green fluorescent protein (GFP) fusion protein and bimolecular fluorescence complementation (BiFC) assay suggested that TatrHb is involved in photosynthesis. The TatrHb-GFP fusion protein was localized in the plastids and the yellow fluorescent protein signal indicated that the TatrHb protein interacted with PSK-I and PsbS1 in the chloroplast.

Additional key words

chloroplast gibberellic acid NaCl Triticum aestivum nitric oxide photosynthesis YIIH assay 

Abbreviations

ABA

abscisic acid

BiFC

bimolecular fluorescence complementation

c-PTIO

2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3 oxidase

GA3

gibberellic acid

GFP

green fluorescent protein

Hb

hemoglobin

MeJA

methyl jasmonate

NOS

NO synthase

ONPG

O-nitrophenyl β-D-galactopyranoside

PEG

polyethylene glycol

RT-PCR

reverse transcriptase-polymerase chain reaction

SA

salicylic acid

SNP

sodium nitroprusside

TatrHb

Triticum aestivum L. truncated hemoglobin

YIIH

yeast 2-hybrid

YFP

yellow fluorescent protein

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Copyright information

© Springer Science+Business Media B.V. 2013

Authors and Affiliations

  • D. Y. Kim
    • 1
  • M. J. Hong
    • 1
  • Y. J. Lee
    • 1
  • M. B. Lee
    • 1
  • Y. W. Seo
    • 1
  1. 1.College of Life Science and BiotechnologyKorea UniversitySeoulKorea

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