Biologia Plantarum

, Volume 57, Issue 1, pp 174–178 | Cite as

The role of cytokinins during micropropagation of wych elm

  • J. Malá
  • P. Máchová
  • H. Cvrčková
  • M. Karady
  • O. Novák
  • J. Mikulík
  • J. Dostál
  • M. Strnad
  • K. Doležal
Brief Communication

Abstract

We have compared the influence of two aromatic cytokinin derivatives, N6-benzyladenine (BA) and meta-topolin (mT), on the in vitro multiplication and senescence of wych elm (Ulmus glabra Huds.). After 3 months of cultivation, the micropropagation rate was higher (approx. six times more shoots developed) on Murashige and Skoog (MS) medium supplemented with mT than on MS supplemented with BA. Quantification of 50 endogenous cytokinins, using a recently developed UPLC-ESI(+)-MS/MS method, showed significant differences in the cytokinin metabolites (especially different glucosides) in explants cultivated on media supplemented with BA or mT. Differences in efficiency of photosystem II was also detected.

Additional key words

benzylaminopurine chlorophyll fluorescence meta-topolin organogenesis rooting senescence 

Abbreviations

BA

N6-benzyladenine

cZ

cis-zeatin

DHZ

dihydrozeatin

Fv/Fm

variable to maximum chlorophyll fluorecence ratio

*9G

9-β-D-glucopyranosyl derivative

IAC

immunoaffinity chromatography

IBA

indole-3-butyric acid

iP

N6-isopentenyladenine

*MP

5′-monophosphate derivative

LC-MS

liquid chromatography combined with mass spectrometry

MS

Murashige and Skoog medium

mT

meta-topolin [6-(3-hydroxybenzylamino)purine]

*OG

O-β-D-glucopyranosyl derivative

oT

ortho-topolin

PS II

photosystem II

*R

9-β-D-ribofuranosyl derivative

tZ

trans-zeatin

UPLCESI(+)-MS/MS

ultra performance liquid chromatography combined with positive electrospray mass spectrometry

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • J. Malá
    • 1
  • P. Máchová
    • 1
  • H. Cvrčková
    • 1
  • M. Karady
    • 2
  • O. Novák
    • 2
  • J. Mikulík
    • 2
  • J. Dostál
    • 1
  • M. Strnad
    • 2
    • 3
  • K. Doležal
    • 2
    • 3
  1. 1.Forestry and Game Management Research InstituteJílovištěCzech Republic
  2. 2.Laboratory of Growth RegulatorsPalacký University and Institute of Experimental Botany AS CROlomouc-HoliceCzech Republic
  3. 3.Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of SciencePalacký UniversityOlomoucCzech Republic

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