Biologia Plantarum

, Volume 57, Issue 1, pp 56–62

Molecular cloning and expression analysis of a new stress-related AREB gene from Arachis hypogaea

  • L. Hong
  • B. Hu
  • X. Liu
  • C. Y. He
  • Y. Yao
  • X. L. Li
  • L. Li


An AREB gene, designated as AhAREB1, was cloned from peanut (Arachis hypogaea L.). The gene contains a 1 338-bp open reading frame that encodes a putative protein of 445 amino acids. The corresponding genomic DNA containing four exons and three introns was isolated and analyzed. An upstream 1 060-bp DNA promoter fragment of the AhAREB1 gene was also amplified from peanut genomic DNA. Multiple sequence alignment of the deduced amino acids of AREB showed that the AhAREB1 protein shares high sequence homology with GmAREB1, S1AREB and ABF2. Quantitative real-time PCR analysis showed that AhAREB1 was induced by polyethylene glycol, NaCl, gibberellic acid, abscisic acid and salicylic acid. The cloning and characterization of the AhAREB1 gene will be useful for further studies establishing the biological role of AhAREB1 in plants.

Additional key words

ABA AhAREB1 GA3 peanut PEG SA water stress 



abscisic acid


ABRE binding factor


ABA response element


ABA-response element binding protein


basic leucine zipper


calmodulin-dependent protein kinase


casein kinase


gibberellic acid


9-cis-epoxycarotenoid dioxygenase


open reading frame


polyethylene glycol


protein kinase C


salicylic acid


WRKY binding site


superfamily of plant transcription factors


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • L. Hong
    • 1
    • 2
  • B. Hu
    • 1
  • X. Liu
    • 1
  • C. Y. He
    • 1
  • Y. Yao
    • 1
  • X. L. Li
    • 1
  • L. Li
    • 1
  1. 1.Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life SciencesSouth China Normal UniversityGuangzhouP.R. China
  2. 2.College of Horticulture and Landscape ArchitectureZhongkai University of Agriculture and EngineeringGuangzhouP.R. China

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