Biologia Plantarum

, Volume 57, Issue 1, pp 33–40 | Cite as

Photosynthetic parameters of Ulmus minor plantlets affected by irradiance during acclimatization

  • M. C. Dias
  • G. Pinto
  • C. M. Correia
  • J. Moutinho-Pereira
  • S. Silva
  • C. Santos


In order to set up large-scale acclimatization protocols of micropropagated plants, an in-depth knowledge of their physiological responses during in vitro to ex vitro transfer is required. This work describes the photosynthetic performance of Ulmus minor micropropagated plants during acclimatization at high irradiance (HI; 200 ± 20 μmol m−2 s−1 or low irradiance (LI; 100 ± 20 μmol m−2 s−1). During this experiment, leaf pigment content, chlorophyll a fluorescence, gas exchange, stomata morphology, the activity of the Calvin cycle enzymes and saccharides were measured in persistent and new leaves. The results indicated that HI induces a higher photosynthetic performance compared to LI. Therefore, plants acclimatized under HI are likely to survive better after field transfer.

Additional key words

Calvin cycle enzymes chlorophyll elm fluorescence micropropagation net photosynthetic rate stomata transpiration rate 







dry mass


ratio of intercellular to atmospheric CO2 concentration


transpiration rate


variable to maximum chlorophyll fluorescence (maximal efficiency of PSII)


stomatal conductance


high irradiance


low irradiance


net photosynthetic rate


photosystem 2


non-photochemical quenching


photochemical quenching


relative humidity


ribulose-1,5-bisphosphate carboxylase/oxygenase


stromal fructose-1,6-bisphosphatase


effective quantum efficiency of PSII


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • M. C. Dias
    • 1
  • G. Pinto
    • 1
  • C. M. Correia
    • 2
  • J. Moutinho-Pereira
    • 2
  • S. Silva
    • 1
  • C. Santos
    • 1
  1. 1.Department of Biology and Centre for Environmental and Marine Studies (CESAM)University of AveiroAveiroPortugal
  2. 2.Centre for Research and Technology in Agro-Environmental and Biological Sciences (CITAB) and Department of Biology and EnvironmentUniversity of Trás-os-Montes e Alto DouroVila RealPortugal

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