Photosynthetic parameters of Ulmus minor plantlets affected by irradiance during acclimatization
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In order to set up large-scale acclimatization protocols of micropropagated plants, an in-depth knowledge of their physiological responses during in vitro to ex vitro transfer is required. This work describes the photosynthetic performance of Ulmus minor micropropagated plants during acclimatization at high irradiance (HI; 200 ± 20 μmol m−2 s−1 or low irradiance (LI; 100 ± 20 μmol m−2 s−1). During this experiment, leaf pigment content, chlorophyll a fluorescence, gas exchange, stomata morphology, the activity of the Calvin cycle enzymes and saccharides were measured in persistent and new leaves. The results indicated that HI induces a higher photosynthetic performance compared to LI. Therefore, plants acclimatized under HI are likely to survive better after field transfer.
Additional key wordsCalvin cycle enzymes chlorophyll elm fluorescence micropropagation net photosynthetic rate stomata transpiration rate
ratio of intercellular to atmospheric CO2 concentration
variable to maximum chlorophyll fluorescence (maximal efficiency of PSII)
net photosynthetic rate
effective quantum efficiency of PSII
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