Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis
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Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis from leaf explants were examined. During the early stages of shoot organogenesis there was a decrease in superoxide dismutase (SOD) and an increase in catalase (CAT) activity, and later during organogenesis there was an increase in peroxidase (POD) and polyphenol oxidase (PPO) activity. Two highly regulated turning points may be distinguished regarding activities and isoforms of antioxidative enzymes: the initiation of shoot organogenesis and the shoot bud formation. Our data suggest the role of specific CAT, POD, SOD and PPO isoforms in separate processes during T. bellus direct shoot organogenesis.
Additional key wordscatalase peroxidase polyphenol oxidase superoxide dismutase
sodium dodecyl sulfate
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This work was supported by a grant (No. 173017 and 173015) from the Ministry of Education and Science of the Republic of Serbia.
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