Biologia Plantarum

, Volume 56, Issue 2, pp 357–361 | Cite as

Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis

  • A. Mitrović
  • D. Janošević
  • S. Budimir
  • J. Bogdanović Pristov
Brief Communication


Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis from leaf explants were examined. During the early stages of shoot organogenesis there was a decrease in superoxide dismutase (SOD) and an increase in catalase (CAT) activity, and later during organogenesis there was an increase in peroxidase (POD) and polyphenol oxidase (PPO) activity. Two highly regulated turning points may be distinguished regarding activities and isoforms of antioxidative enzymes: the initiation of shoot organogenesis and the shoot bud formation. Our data suggest the role of specific CAT, POD, SOD and PPO isoforms in separate processes during T. bellus direct shoot organogenesis.

Additional key words

catalase peroxidase polyphenol oxidase superoxide dismutase 







ethylenediaminetetraacetic acid


naphtaleneacetic acid




polyphenol oxidase




superoxide dismutase


sodium dodecyl sulfate




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This work was supported by a grant (No. 173017 and 173015) from the Ministry of Education and Science of the Republic of Serbia.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • A. Mitrović
    • 1
  • D. Janošević
    • 3
  • S. Budimir
    • 2
  • J. Bogdanović Pristov
    • 1
  1. 1.Institute for Multidisciplinary ResearchUniversity of BelgradeBelgradeSerbia
  2. 2.Institute for Biological Research “Siniša Stanković”University of BelgradeBelgradeSerbia
  3. 3.Institute of Botany and Botanical Garden „Jevremovac“University of BelgradeBelgradeSerbia

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