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Biologia Plantarum

, Volume 56, Issue 2, pp 321–329 | Cite as

Hydrogen peroxide and nitric oxide promote reproductive growth in Litchi chinensis

  • B. ZhouEmail author
  • N. Li
  • Z. Zhang
  • X. Huang
  • H. Chen
  • Z. Hu
  • X. Pang
  • W. Liu
  • Y. Lu
Original Papers

Abstract

Vegetative growth and reproductive growth strongly competes with each other during panicle development in litchi (Litchi chinensis Sonn.). We herein investigated the roles of hydrogen peroxide and nitric oxide in the competition between growth of rudimentary leaves and panicle development. The results show that the chilling-induced flowering increased H2O2 and NO contents in the mixed buds. Treatments with sodium nitroprusside (SNP), the NO donor, and methyl viologen dichloride hydrate (MV), the superoxide generator, increased NO and H2O2 contents in the mixed buds. MV and SNP treatments promoted abscission of rudimentary leaves and encouraged panicle development before or at the stage of panicle emergence. The nitric oxide synthase inhibitor N ω -nitro-L-arginine methyl ester (L-NAME) and the H2O2 trapper dimethylthiourea (DMTU) inhibited a chilling-induced flowering. SNP promoted the expression of litchi LEAFY homolog (LcLFY). These promotive effects were suppressed by the NO scavenger, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl3-oxide (PTIO) and the H2O2 trapper, DMTU. The results suggest that H2O2 and NO promote reproductive growth by inhibiting the growth of rudimentary leaves as well as by promoting the expression of the flower related gene, LcLFY.

Additional key words

flowering LEAFY methyl viologen sodium nitroprusside 

Abbreviations

ABA

abscisic acid

4AF-DA

4-aminofluorescin diacetate

DAF-2DA

diaminofluorescein diacetate

DCFH-DA

dichlorofluorescin diacetate

DMTU

dimethylthiourea

LcLFY

LEAFY homolog

L-NAME

N ω -nitro-L-arginine methyl ester

MV

methyl viologen dichloride hydrate

PTIO

2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide

SNP

sodium nitroprusside

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Notes

Acknowledgements

We thank P. O’Malley and Y.Q. O’Malley for proofreading. This study was funded by the National Natural Science Foundation (30571283, 31071760), 948 Projects of Agricultural Ministry (2006G31), and the Agricultural Industry Project (nyhyzx07-031) by the Ministry of Agriculture.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • B. Zhou
    • 1
    Email author
  • N. Li
    • 1
  • Z. Zhang
    • 1
  • X. Huang
    • 1
  • H. Chen
    • 1
  • Z. Hu
    • 1
  • X. Pang
    • 2
  • W. Liu
    • 1
  • Y. Lu
    • 1
  1. 1.College of HorticultureSouth China Agricultural UniversityGuangzhouP.R. China
  2. 2.College of Life ScienceSouth China Agricultural UniversityGuangzhouP.R. China

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