Biologia Plantarum

, Volume 56, Issue 3, pp 545–550 | Cite as

Chalcone isomerase in grape vine: gene expression and localization in the developing fruit

  • W. Wang
  • H. -L. Wang
  • S. -B. Wan
  • J. -H. Zhang
  • P. Zhang
  • J. -C. Zhan
  • W. -D. Huang


Chalcone isomerase (CHI, EC is an entrance enzyme in the flavonoid biosynthesis, which catalyzes the conversion of chalcones to flavanones. In this study, the full-length CHI cDNA from grape vine (Vitis vinifera L.) was cloned, the recombinant protein was purified and the polyclonal antibody was prepared. Using these tools, the expression and tissue localization of CHI in developing grape berry was analyzed by RT-PCR, gel blot hybridization and immunohistochemical techniques. The expression of CHI was dependent on developmental stage, and CHI protein was mainly distributed in vascular bundles throughout all the stages of berry development, which suggested that flavonoids in the berry might have been partially synthesized in situ.

Additional key words

antibody Escherichia coli; flavonoids immunohistochemical technique RT-PCR Vitis vinifera 



bovine serum albumin


chalcone isomerase


chalcone synthase


day after full bloom


ethylene diamine tetraacetic acid




reverse transcription-polymerase chain reaction


sodium dodecyl sulfate


tris(hydroxymethyl)amino methane


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • W. Wang
    • 1
    • 3
  • H. -L. Wang
    • 1
    • 2
  • S. -B. Wan
    • 1
    • 4
  • J. -H. Zhang
    • 1
  • P. Zhang
    • 1
  • J. -C. Zhan
    • 1
  • W. -D. Huang
    • 1
    • 2
  1. 1.Center for Research and Development of Viticulture and EnologyChina Agricultural UniversityBeijingP.R. China
  2. 2.Beijing Key Laboratory of Viticulture and EnologyBeijingChina
  3. 3.The Institute of ForestryThe Chinese Academy of ForestryBeijingP.R. China
  4. 4.School of Life ScienceShanghai UniversityShanghaiP.R. China

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