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Biologia Plantarum

, Volume 55, Issue 3, pp 577–580 | Cite as

Comparison of genomic SSR and EST-SSR markers for estimating genetic diversity in cucumber

  • J. Hu
  • L. Wang
  • J. LiEmail author
Brief Communication

Abstract

Thirteen genomic microsatellite (gSSR) and sixteen expressed sequence tag (EST)-SSR (eSSR) markers were compared to estimate genetic diversity among 29 cucumber (Cucumis sativus L.) accessions. gSSR markers detected mean 4.46 alleles with a mean polymorphic information content (PIC) of 0.664, against eSSR markers with mean 3.38 alleles and a mean PIC of 0.397. gSSRs amplified more null alleles than eSSRs. Genetic diversity within the accession set was estimated by construction of dendrograms using gSSR or eSSR data. There was a clear consistency between gSSR and eSSR trees in terms of positioning of most cucumber germplasms. gSSR markers could separate various types of cucumber germplasms on the whole, although clustering of some accessions was not based on their geographical origins in eSSR tree. eSSR markers identified an independent sub-cluster containing five accessions resistant to downy mildew, suggesting a probable relationship between eSSRs and disease-resistance trait in cucumber. The Mantel test between gSSR and eSSR matrices revealed a good fit correlation (r = 0.836). The general dendrogram constructed using the combined data of gSSRs and eSSRs was similar to those obtained separately with each marker.

Additional key words

Cucumis sativus dendrograms polymorphic information content 

Abbreviations

EG, EO, NC, OP, SC

Europe greenhouse, Europe open-field

north China

occident processing and south China accessions, respectively

eSSR

expressed sequence tag microsatellite

EST

expressed sequence tag

gSSR

genomic microsatellite

PIC

polymorphic information content

UPGMA

unweighted pair group method using arithmetic averages

W

wild species

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Notes

Acknowledgements

This work is supported by National Key Technology R & D Program (No. 2009BADB8B02). We are grateful to Prof. Jiancan Feng, College of Horticulture, Henan Agricultural University, for his assistance in the laboratory work on this project.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Open Key Laboratory of Horticultural Plant Physiology and Genetic Improvement, High School of Henan Province; Key Laboratory of Fruit Plant and Cucurbit, Henan Province; College of HorticultureHenan Agricultural UniversityZhengzhouP.R. China

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