Biologia Plantarum

, Volume 55, Issue 3, pp 528–535

Special origin of stem sequence influence the resistance of hairpin expressing plants against PVY

  • F. Jiang
  • B. Wu
  • C. Zhang
  • Y. Song
  • H. An
  • C. Zhu
  • F. Wen
Original Papers

Abstract

In this study, 16 hairpin RNA (hpRNA) vectors were constructed, each harboring 50 bp viral RNA sequence as the stem. They all targeted the coat protein (CP) gene of Potato virus Y (PVY). Virus resistance assay revealed that hairpin constructs targeting the anterior 200 bp regions of the CP gene were unable to induce virus resistance, while the 12 hpRNA constructs targeting posterior 600 bp regions induced high virus resistance up to 77.78 %. Northern blot analysis revealed that 50 bp-length hpRNA constructs could be transcribed efficiently and processed into siRNAs; however, no correlation between siRNA accumulation and degree of antiviral defense was observed. Results presented here indicated that the middle and 3′ end of the CP cDNA was important for hpRNA-mediated PVY resistance, improving the design of pathogen-derived hpRNA expression cassettes for transgenic plant against viruses.

Additional key words

Agrobacterium tumefaciens hpRNA-mediated virus resistance Nicotiana tabacum RNA silencing 

Abbreviations

CaMV

cauliflower mosaic virus

CB

carbenicillin

CP

coat protein

dpi

day post-inoculation

dsRNA

double strand RNA

hpRNA

hairpinRNA

PTGS

post-transcriptional gene silencing

PVY

Potato virus Y

SSC

sodium chloride/sodium citrate

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • F. Jiang
    • 1
  • B. Wu
    • 1
  • C. Zhang
    • 1
  • Y. Song
    • 1
  • H. An
    • 1
  • C. Zhu
    • 1
  • F. Wen
    • 1
  1. 1.State Key Laboratory of Crop Biology, College of Life SciencesShandong Agricultural UniversityTaian, ShandongP.R. China

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