Biologia Plantarum

, Volume 55, Issue 2, pp 340–344 | Cite as

Effects of salt and osmotic stresses on free polyamine content and expression of polyamine biosynthetic genes in Vitis vinifera

Brief Communication

Abstract

Grape (Vitis vinifera L.) seedlings grown in vitro were treated with either 200 mM NaCl or 350 mM mannitol for 7 d. Both salinity and osmotic stress caused significant increase in electrolyte leakage. From the three commonly occurring free polyamines (PA), only conspicuous accumulation of putrescine was found in the NaCl-treated seedlings. Four PA biosynthetic genes encoding arginine decarboxylase (pVvADC), S-adenosylmethionine decarboxylase (pVvSAMDC), spermidine synthase (pVvSPDS) and spermine synthase (pVvSPMS) were successfully isolated. While induction of pVvADC was observed from the 1st day of salt treatment, pVvSAMDC and pVvSPMS were induced only at late stage of stress. As for expression levels of genes in the mannitol-treated seedling, either temporary (pVvADC at day 1) or late (pVvSPMS at days 5 and 7) induction was observed.

Additional key words

abiotic stress electrolyte leakage grape mannitol NaCl putrescine spermine spermidine 

Abbreviations

ADC

arginine decarboxylase

EL

electrolyte leakage

MS

Murashige and Skoog

ODC

ornithine decarboxylase

Put

putrescine

SAMDC

S-adenosylmethionine decarboxylase

Spd

spermidine

SPDS

spermidine synthase

Spm

spermine

SPMS

spermine synthase

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Notes

Acknowledgements

This work was supported in part by Grants-in-Aid from Japan Society for Promotion of Science (JSPS).

References

  1. Aghaleh, M., Niknam, V., Ebrahimzadeh, H., Razavi, K.: Salt stress effects on growth, pigments, proteins and lipid peroxidation in Salicornia persica and S. europaea. — Biol. Plant. 53: 243–248, 2009.CrossRefGoogle Scholar
  2. Aziz, A.: Spermidine and related-metabolic inhibitors modulate sugar and amino acid levels in Vitis vinifera L.: possible relationships with initial fruitlet abscission. — J. exp. Bot. 54: 355–363, 2003.PubMedCrossRefGoogle Scholar
  3. Aziz, A., Brun, O., Audran, J.C.: Involvement of polyamines in the control of fruitlet physiological abscission in grapevine (Vitis vinifera). — Physiol. Plant. 113: 50–58, 2001.CrossRefGoogle Scholar
  4. Bertoldi, D., Tassoni, A., Martinelli, L., Bagni, N.: Polyamine and somatic embryogenesis in two Vitis vinefera cultivars. — Physiol. Plant. 120: 657–666. 2004.PubMedCrossRefGoogle Scholar
  5. Botella, M.Á., Del Amor, F., Amorós, A., Serrano, M., Martínez, V., Cerdá, A.: Polyamine, ethylene and other physico-chemical parameters in tomato (Lycopersicon esculentum) fruits as affected by salinity. — Physiol. Plant. 109: 428–434, 2000.CrossRefGoogle Scholar
  6. Farooq, M., Wahid, A., Lee, D.J.: Exogenously applied polyamines increase drought tolerance of rice by improving leaf water status, photosynthesis and membrane properties. — Acta Physiol. Plant. 31: 937–945, 2009.CrossRefGoogle Scholar
  7. Flowers, T. J.: Improving crop salt tolerance. — J. exp. Bot. 396: 307–319, 2004.CrossRefGoogle Scholar
  8. Kitashiba, H., Hao, Y.J., Honda, C., Moriguchi, T.: Two types of spermine synthase gene: MdACL5 and MdSPMS are differentially involved in apple fruit development and cell growth. — Gene 361: 101–111, 2005.PubMedCrossRefGoogle Scholar
  9. Kusano, T., Berberich, T., Tateda, C., Takahashi, Y.: Polyamines: essential factors for growth and survival. — Planta 228: 367–381, 2008.PubMedCrossRefGoogle Scholar
  10. Liu, J.H., Ban, Y., Wen, X.P., Nakajima, I., Moriguchi, T.: Molecular cloning and expression analysis of an arginine decarboxylase gene from peach (Prunus persica). — Gene 429: 10–17, 2009.PubMedCrossRefGoogle Scholar
  11. Liu, J.H., Kitashiba, H., Wang, J., Ban, Y., Moriguchi, T.: Polyamines and their ability to provide environmental stress tolerance to plants. — Plant Biotechnol. 24: 117–126, 2007.CrossRefGoogle Scholar
  12. Liu, J.H., Moriguchi, T.: Changes in free polyamine titers and expression of polyamine biosynthetic genes during growth of peach in vitro callus. — Plant Cell Rep. 26: 125–131, 2007.PubMedCrossRefGoogle Scholar
  13. Liu, J.H., Moriguchi, T.: Salt stress-mediated changes in free polyamine titers and expression of genes responsible for polyamine biosynthesis of apple in vitro shoots. — Environ. exp. Bot. 62: 28–35, 2008.CrossRefGoogle Scholar
  14. Liu, J.H., Nada, K., Honda, C., Kitashiba, H., Wen, X.P., Pang, X.M., Moriguchi, T.: Polyamine biosynthesis of apple callus under salt stress: importance of arginine decarboxylase pathway in stress response. — J. exp. Bot. 57: 2589–2599, 2006b.PubMedCrossRefGoogle Scholar
  15. Liu, J.H., Nada, K., Pang, X.M., Honda, C., Kitashiba, H., Moriguchi, T.: Role of polyamines in peach fruit development and storage. — Tree Physiol. 26: 791–798. 2006a.PubMedGoogle Scholar
  16. Lutts, S., Almansouri, M., Kinet, J.M.: Salinity and water stress have contrasting effects on the relationship between growth and cell viability during and after stress exposure in durum wheat callus. — Plant Sci. 167: 9–18, 2004.CrossRefGoogle Scholar
  17. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.CrossRefGoogle Scholar
  18. Primikirios, N.I., Roubelakis-Angelakis, K.A.: Cloning and expression of an arginine decarboxylase cDNA from Vitis vinifera L. cell-suspension cultures. — Planta 208: 574–582, 1999.PubMedCrossRefGoogle Scholar
  19. Santa-Cruz, A., Acosta, M., Pérez-Alfocea, F., Bolarin, M.C.: Changes in free polyamine levels induced by salt stress in leaves of cultivated and wild tomato species. — Physiol. Plant. 101: 341–346, 1997.CrossRefGoogle Scholar
  20. Shinozaki, K., Yamaguchi-Shinozaki, K.: Gene networks involved in drought stress response and tolerance. — J. exp. Bot. 58: 221–227, 2007.PubMedCrossRefGoogle Scholar
  21. Tassoni, A., Franceschetti, M., Tasco, G., Casadio, R., Bagni, N.: Cloning, functional identification and structural modelling of Vitis vinifera S-adenosylmethionine decarboxylase. — J. Plant Physiol. 164: 1208–1219, 2007.PubMedCrossRefGoogle Scholar
  22. Tonon, G., Kevers, C., Faivre-Rampant, O., Grazianil, M., Gaspar, T.: Effect of NaCl and mannitol iso-osmotic stresses on proline and free polyamine levels in embryogenic Fraxinus angustifolia callus. — J. Plant Physiol. 161: 701–708, 2004.PubMedCrossRefGoogle Scholar
  23. Urano, K., Yoshiba, Y., Nanjo, T., Igarashi, Y., Seki, M., Sekiguchi, F., Yamaguchi-Shinozaki, K., Shinozaki, K.: Characterization of Arabidopsis genes involved in biosynthesis of polyamines in abiotic stress response and developmental stages. — Plant Cell Environ. 26: 1917–1926, 2003.CrossRefGoogle Scholar
  24. Urano, K., Yoshiba, Y., Nanjo, T., Ito, Y., Seki, M., Yamaguchi-Shinozaki, K., Shinozaki, K.: Arabidopsis stress-inducible gene for arginine decarboxylase AtADC2 is required for accumulation of putrescine in salt tolerance. — Biochem. Biophys. Res. 313: 369–375, 2004.CrossRefGoogle Scholar
  25. Verma, S., Mishra, S.N.: Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense system. — J. Plant Physiol. 162, 669–677, 2005.PubMedCrossRefGoogle Scholar
  26. Wan, C.Y., Wilkins, T.A.: A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.). — Anal. Biochem. 223: 7–12, 1994.PubMedCrossRefGoogle Scholar
  27. Wi, S.J., Kim, W.T., Park, K.Y.: Overexpression of carnation S-adenosylmethionine decarboxylase gene generates a broad-spectrum tolerance to abiotic stresses in transgenic tobacco plants. — Plant Cell Rep. 25: 1111–1121, 2006.PubMedCrossRefGoogle Scholar
  28. Zhu, J.K.: Plant salt tolerance. — Trends Plant Sci. 6: 66–71, 2001.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.National Key Laboratory of Crop Genetic Improvement, Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanP.R. China
  2. 2.National Institute of Fruit Tree ScienceTsukuba, IbarakiJapan

Personalised recommendations