Biologia Plantarum

, Volume 55, Issue 2, pp 317–322

Pseudomonas fluorescens mediated systemic resistance in tomato is driven through an elevated synthesis of defense enzymes

Article

DOI: 10.1007/s10535-011-0045-3

Cite this article as:
Vanitha, S.C. & Umesha, S. Biol Plant (2011) 55: 317. doi:10.1007/s10535-011-0045-3

Abstract

Pseudomonas fluorescens was used as a biological control agent against bacterial wilt disease caused by Ralstonia solanacearum. Activities of phenylalanine ammonia lyase (PAL), guaiacol peroxidase (POX), polyphenol oxidase (PPO) and lipoxygenase (LOX) increased in P. fluorescens pretreated tomato seedlings, which were later inoculated with R. solanacearum and activities of PAL, POX, PPO and LOX reached maximum at 12, 9, 15 and 9 h, respectively, after inoculation. Reverse transcription — polymerase chain reaction (RT-PCR) confirmed the maximum induction of all these enzymes in P. fluorescens pretreated seedlings.

Additional key words

gene expression lipoxygenase Lycopersicon esculentum peroxidase phenylalanine ammonia lyase polyphenol oxidase Ralstonia solanacearum 

Abbreviations

CA

trans-cinnamic acid

ISR

induced systemic resistance

LOX

lipoxygenase

PAL

phenylalanine ammonia lyase

PGPR

plant growth promoting rhizobacteria

POX

guaiacol peroxidase

PPO

polyphenol oxidase

RT-PCR

reverse transcription — polymerase chain reaction

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Department of Studies in BiotechnologyUniversity of MysoreManasagangotri, MysoreIndia

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