Abstract
The unusual glandular trichomes of willow-herbs (Epilobium hirsutum L.) were investigated. They are unicellular, without a specialized basal cell at the layer of the epidermis. They have a cutinized cell wall, a prominent and heavily cutinized wall thickening at the base, and a protruding pore on the top. Among other compounds they contain several flavonoids, e.g. quercitrin and myricitrin. Flavonoids were localized inside the upper part of the trichome cell and most likely were not excreted through the trichome pore.
References
Agati, G., Galardi, C., Gravano, E., Romani, A., Tattini, M.: Flavonoid distribution in tissues of Phillyrea latifolia L. leaves as estimated by microspectrofluorometry and multispectral fluorescence microimaging. — Photochem. Photobiol. 76: 350–360, 2002.
Croteau, R., Kutchan, T.M., Lewis, N.G.: Natural products. — In: Buchanan, B.B., Gruissem, W., Jones, R.L. (ed.): Biochemistry and Molecular Biology of Plants. Pp. 1250–1318. American Society of Plant Physiologists, Rockville 2000.
Ducrey, B., Marston, A., Göhring, S., Hartmann, R.W., Hostettmann, K.: Inhibition of 5α-reductase and aromatase by the ellagitannins oenotein A and oenothein B from Epilobium species. — Planta med. 63: 111–114, 1997.
Ducrey, B., Wolfender, J.L., Marston, A., Hostettmann, K.: Analysis of flavonol glycosides of thirteen Epilobium species (Onagraceae) by LC-UV and thermospray LC-MS. — Phytochemistry 38: 129–137, 1995.
Fahn, A., Shimony, C.: Glandular trichomes of Fagonia L. (Zygophyllaceae) species: structure, development and secreted materials. — Ann. Bot. 77: 25–34, 1996.
Fico, G., Rodondi, G., Flamini, G., Passarella, D., Tome, F.: Comparative phytochemical and morphological analyses of three Italian Primula species. — Phytochemistry 68: 1683–1691, 2007.
Haussknecht, C.: Monographie der Gattung Epilobium. — Gustav Fischer Verlag, Jena 1884.
Heinrich, G., Pfeifhofer, H.W., Stabentheiner, E., Sawidis, T.: Glandular hairs of Sigesbeckia jorullensis Kunth (Asteraceae): morphology, histochemistry and composition of essential oil. — Ann. Bot. 89: 459–469, 2002.
Jork, H., Funk W., Fischer W., Wimmer H.: Dünnschicht-Chromatographie, Reagenzien und Nachweismethoden. Band 1a. — VCH Verlagsgesellschaft, Weinheim 1989.
Kladnik, A., Chamusco, K., Dermastia, M., Chourey, P.: Evidence of programmed cell death in post-phloem transport cells of the maternal pedicel tissue in developing caryopsis of maize. — Plant Physiol. 136: 3572–3581, 2004.
Kostina, E., Wulff, A., Julkunen-Tiitto, R.: Growth, structure, stomatal responses and secondary metabolites of birch seedlings (Betula pendula) under elevated UV-B radiation in the field. — Trees 15: 483–491, 2001.
Rautio, P., Markkola, A., Martel, J., Tuomi, J., Härmä, E., Kuikka, K., Siitonen, A., Riesco, I.L., Roitto, M.: Developmental plasticity in birch leaves: defoliation causes a shift from glandular to nonglandular trichomes. — Oikos 98: 437–446, 2002.
Spring, O.: Chemotaxonomy based on metabolites from glandular trichomes. — Adv. Bot. Res. 31: 153–174, 2000.
Strgulc Krajšek, S., Dermastia, M., Jogan, N.: Determination key for Central European Epilobium species based on trichome morphology. — Bot. helvet. 116: 169–178, 2006.
Tattini, M., Gravano, E., Pinelli, P., Mulinacci, N., Romani, A.: Flavonoids accumulate in leaves and glandular trichomes of Phillyrea latifolia exposed to excess solar radiation. — New Phytol. 148: 69–77, 2000.
Tattini, M., Guidi, L., Morassi-Bonsi, L., Pinelli, P., Remorini, D., Degl’Innocenti, E., Giordano, C., Massai, R., Agati, G.: On the role of flavonoids in the integrated mechanisms of response of Ligustrum vulgare and Phillyrea latifolia to high solar radiation. — New Phytol. 167: 457–470, 2005.
Toth, H.B., Balazs, A., Vukics, V., Szoeke, E., Kery, A.: Identification of Epilobium species and willow-herbs (Onagraceae) by HPLC analysis of flavonoids as chemotaxonomic markers. — Chromatography 63(Suppl.): S119–S123, 2006.
Uphof, J.C.T.: Plant hairs. — In: Zimmermann, W., Ozenda, P.G. (ed.): Encyclopedia of Plant Anatomy. Pp. 1–206. Bornträger, Berlin 1962.
Wagner, H., Bladt, S., Zgainski, E.M.: Drogenanalyse. Dünnschichtchromatographische Analyse von Arzneidrogen. Pp. 299–305. Springer-Verlag, Berlin 1983.
Yang, Y., He, F., Yu, L.J.: Dynamics analyses of nutrients consumption and flavonoids accumulation in cell suspension culture of Glycyrrhiza inflate. — Biol. Plant. 52: 732–734, 2008.
Acknowledgements
This work was supported by the Ministry of Education, Science and Sport, Republic of Slovenia (grant No. S4-487-001/212410/2000 awarded to S. S. K.) and the Slovenian Research Agency (grant No. P1-0212).
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Strgulc Krajšek, S., Kreft, S., Kladnik, A. et al. Morphology and glandular activity of unicellular trichomes of Epilobium hirsutum . Biol Plant 55, 149–152 (2011). https://doi.org/10.1007/s10535-011-0020-z
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DOI: https://doi.org/10.1007/s10535-011-0020-z