Biologia Plantarum

, Volume 54, Issue 4, pp 601–606 | Cite as

Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

Original Papers

Abstract

The effect of plant growth regulators (PGRs), gelling agents, sucrose and heat shock on shoot multiplication, shoot growth, rooting and subsequent survival of Chlorophytum borivilianum Sant. et Fernand was evaluated. Benzyladenine (BA) was found to be better cytokinin over kinetin (KIN) for shoot multiplication. Sucrose concentrations from 116–290 mM in the basal medium (BM) promoted shoot multiplication. Heat shock (50 °C, 1 h) also promoted shoot multiplication at these sucrose concentrations on both BM medium and BM supplemented with 5.0 μM BA. Beneficial effect of sucrose was also observed on rooting of shoots on BM as well as BM supplemented with 5.0 μM indole-3-butyric acid (IBA). Phytagel as a gelling agent was found to be more effective for shoot proliferation and growth compared to agar. Amongst various soil mixtures tested, higher survival of plants was observed in soil containing Vermicompost. It was interesting to note that a maximum plant survival (> 95 %) was observed when plants were directly transferred to net-house (irradiance reduced to 50 % with green net, without humidity and temperature control) than poly-house (with humidity and temperature control). Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analysis of regenerated plants showed genetic similarity to mother plant.

Additional key words

acclimatization heat shock ISSR micropropagation RAPD sucrose 

Abbreviations

BA

benzyladenine

BAM

basal MS medium supplemented with 5.0 μM BA

BIBA

basal MS medium supplemented with 5.0 μM IBA

BM

basal MS medium

HS

heat shock

IBA

indole-3-butyric acid

ISSR

inter simple sequence repeat

KIN

kinetin

PGRs

plant growth regulators

PCR

polymerase chain reaction

RAPD

random amplified polymorphic DNA

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chandrika, M., Thoyajaksha, Ravishankar Rai, V., Ramachandra Kini, K.: Assesment of genetic stability of in vitro grown Dictyospermum ovalifolium. — Biol. Plant. 52: 735–739, 2008.CrossRefGoogle Scholar
  2. Cline, M., Wessel, T., Iwamura, H.: Cytokinin/auxin control of apical dominance in Ipomoea nill. — Plant Cell Physiol. 38: 659–667, 1997.Google Scholar
  3. Darokar, M.P., Khanuja, S.P.S., Shasany, A.K., Kumar, S.: Low levels of genetic diversity detected by RAPD analysis in geographically distinct accessions of Bacopa monnieri. — Genetic Resour. Crop Evol. 48: 555–558, 2001.CrossRefGoogle Scholar
  4. Dave, A., Bilochi, G., Purohit, S.D.: Scale-up production and field performance of micropropagated medicinal herb ’safed Musli’ (Chlorophytum borivilianum). — In Vitro cell. dev. Biol. Plant. 39: 419–424, 2003.CrossRefGoogle Scholar
  5. Dave, A., Joshi, N., Purohit, S.D.: In vitro propagation of Chlorophytum borivilianum using encapsulated shoot buds. — Eur. J. hort. Sci. 69: 37–42, 2004.Google Scholar
  6. Dave, A., Purohit, S.D.: Factors influencing in vitro culture of ’safed Musli’ (Chlorophytum borivilianum). — Oikoassay 15: 19–27, 2002.Google Scholar
  7. De Bruyn, M.H., Ferreira, D.I.: In vitro corm production of Gladiolus dalenii and G. tristis. — Plant Cell Tissue Organ Cult. 31: 123–128, 1992.CrossRefGoogle Scholar
  8. Debergh, P.C., Maene, L.: Pathological and physiological problems related to the in vitro culture of plants. — Parasitica 40: 69–75, 1984.Google Scholar
  9. Doyle, J.J., Doyle, J.L.: Isolation of plant DNA from the fresh tissue. — Focus 12: 13–15, 1990.Google Scholar
  10. Gopal, J., Iwama, K., Jitsuyama, Y.: Effect of water stress mediated through agar on in vitro growth of potato. — In Vitro cell. dev. Biol. Plant. 44: 221–228, 2008.CrossRefGoogle Scholar
  11. Gupta, P.K., Varshney, R.K.: Molecular markers for genetic fidelity during micropropagation and conservation. — Curr. Sci. 76: 1308–1310, 1999.Google Scholar
  12. Haissig, B.E.: Carbohydrate and amino acid concentration during adventitious root primordium development in Pinus banksiana Lamb. cuttings. — Forest. Sci. 61: 13–19, 1982.Google Scholar
  13. Joshi, P., Dhawan, V.: Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay. — Biol. Plant. 51: 22–26, 2007.CrossRefGoogle Scholar
  14. Kothari, S.K., Singh, K.: Production techniques for the cultivation of safed musli (Chlorophytum borivilianum). — J. Hort. Sci. Biotechnol. 78: 261–264, 2003.Google Scholar
  15. Kumar, A.: Studies on in vitro propagation, biochemistry and field evalution of two economically important plants: Rosa damascene Mill. and Gladiolus spp. — Ph.D. Thesis, Kumaun University, Nainital 1996.Google Scholar
  16. Kumar, A., Palni, L.M.S., Nandi, S.K.: The effect of light source and gelling agent on micro propagation of Rosa damascena Mill. and Rhynchostylis retusa (L.) Bl. — J. Hort. Sci. Biotechnol. 78: 786–792, 2003.Google Scholar
  17. Kumar, A., Palni, L.M.S., Sood, A., Sharma, M., Palni, U.T., Gupta, A.K.: Heat shock induced somatic embryogenesis in callus cultures of gladiolus in the presence of high sucrose. - J. Hort. Sci. Biotechnol. 77: 3–78, 2002.Google Scholar
  18. Kumar, A., Sood, A., Palni, L.M.S. Gupta, A.K.: In vitro propagation of Gladiolus hybridus Hort.: synergistic effect of heat shock and sucrose on morphogenesis. — Plant Cell Tissue Organ Cult. 57: 105–112, 1999.CrossRefGoogle Scholar
  19. Lattoo, S.K., Bamotra, S., Dhar, B.S., Khan, S., Dhar, A.K.: Rapid plant regeneration and analysis of genetic fidelity of in vitro derived plants of Chlorophytum arundinaceum Baker — an endangered medicinal herb. — Plant Cell Rep. 25: 499–506, 2006.CrossRefPubMedGoogle Scholar
  20. Maiti, S., Geetha, K.A.: Characterization, genetic improvement and cultivation of Chlorophytum borivilianum — an important medicinal plant of India. — Plant Genet. Resour. 3: 264–272, 2005.CrossRefGoogle Scholar
  21. Mathur, A., Mathur, A.K., Verma, P., Yadav, S., Gupta, M.L., Darokar, M.P.: Biological hardening and genetic fidelity testing of micro-cloned progeny of Chlorophytum borivilianum Sant. et Fernand. — Afr. J. Biotechnol. 7: 1046–1053, 2008.Google Scholar
  22. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue culture. — Physiol. Plant. 15: 473–497, 1962.CrossRefGoogle Scholar
  23. Nairn, B.: Significance of gelling agents in a production tissue culture laboratory. — Comb. Proc. Int. Plant Propag. Soc. 37: 200–205, 1988.Google Scholar
  24. Nanda, K.K., Purohit, A.N., Mehrotra, K.: Effect of sucrose, auxins and gibberelic acid on rooting of the stem segments of Populus nigra under varying light conditions. — Plant Cell Physiol. 9: 725–743, 1968.Google Scholar
  25. Narasimham, K.R.S.L., Ravuru, B.K.: A note on some endangered medicinal plants as NTFPs of Eastern Ghats, Andhra Pradesh. — EPTRI-ENVIS Newslett. 9: 11–12, 2003.Google Scholar
  26. Purohit, S.D., Dave, A., Bilochi, G., Joshi, N.: Opportunities for scaling-up production and commercial cultivation of micropropagated safed musli (Chlorophytum borivilianum). — In: Mathur, A.K., Dwivedi, S., Patra, D.D., Bagehi, G.D., Sangwan, N.S., Sharma, A., Khanuja, S.P.S. (ed.): Proceedings of First National Interactive Meeting on Medicinal and Aromatic Plants. Pp. 387–398. CIMAP, Lucknow 2003.Google Scholar
  27. Purohit, S.D., Dave, A., Kukda, G.: Micropropagation of safed musli (Chlorophytum borivilianum), a rare Indian medicinal herb. — Plant Cell Tissue Organ Cult. 39: 93–96, 1994a.CrossRefGoogle Scholar
  28. Purohit, S.D., Dave, A., Kukda, G.: Somatic embryogeny and plantlet regeneration in safed musli (Chlorophytum borivilianum). — Indian J. Plant Genet. Resour. 7: 65–71, 1994b.Google Scholar
  29. Rahman, S.M., Hoasian, A.K.M., Rafiul, I., Joarder, O.I.: Effect of media composition and culture condition on in vitro rooting of rose. — Sci. Hort. 52: 163–169, 1992.CrossRefGoogle Scholar
  30. Rao, M.S., Purohit, S.D.: In vitro shoot but differentiation and plantlet regeneration in Celastrus paniculatus Willd. — Biol. Plant. 50: 501–506, 2006.CrossRefGoogle Scholar
  31. Rizvi, M.Z., Kukreja, A.K., Khanuja, S.P.S.: In vitro culture of Chlorophytum borivilianum Sant. et Fernand in liquid culture medium as a cost effective measure. — Curr. Sci. 92: 87–90, 2007.Google Scholar
  32. Rout, G.R., Mahato, A., Senapati, S.K.: In vitro clonal propagation of Nyctanthes arbor-tristis. — Biol. Plant. 52: 521–524, 2008.CrossRefGoogle Scholar
  33. Schnall, J.A., Hwang, C.H., Cooke, T.J., Zimmerman, J.L.: An evaluation of gene expression during somatic embryogenesis of two temperature sensitive carrot variants unable to complete embryo development. — Physiol. Plant. 82: 498–504, 1991.CrossRefGoogle Scholar
  34. Sharma, N.: Micropropagation of Bacopa monneiri L. Penn. — an important medicinal plant. — M.Sc. Thesis, Thapar University, Patiala 2005.Google Scholar
  35. Tyagi, P., Khanduja, S., Kothari, S.L.: In vitro culture of Capparis decidua and assessment of clonal fidelity of the regenerated plants. — Biol. Plant. 54: 126–130, 2010.CrossRefGoogle Scholar
  36. Vierling, E.: The role of heat shock proteins in plants. — Annu. Rev. Plant Physiol. mol. Biol. 42: 579–620, 1991.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Centre of Relevance and Excellence in Agro and Industrial BiotechnologyThapar UniversityPatialaIndia

Personalised recommendations