Biologia Plantarum

, Volume 54, Issue 4, pp 601–606 | Cite as

Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

Original Papers


The effect of plant growth regulators (PGRs), gelling agents, sucrose and heat shock on shoot multiplication, shoot growth, rooting and subsequent survival of Chlorophytum borivilianum Sant. et Fernand was evaluated. Benzyladenine (BA) was found to be better cytokinin over kinetin (KIN) for shoot multiplication. Sucrose concentrations from 116–290 mM in the basal medium (BM) promoted shoot multiplication. Heat shock (50 °C, 1 h) also promoted shoot multiplication at these sucrose concentrations on both BM medium and BM supplemented with 5.0 μM BA. Beneficial effect of sucrose was also observed on rooting of shoots on BM as well as BM supplemented with 5.0 μM indole-3-butyric acid (IBA). Phytagel as a gelling agent was found to be more effective for shoot proliferation and growth compared to agar. Amongst various soil mixtures tested, higher survival of plants was observed in soil containing Vermicompost. It was interesting to note that a maximum plant survival (> 95 %) was observed when plants were directly transferred to net-house (irradiance reduced to 50 % with green net, without humidity and temperature control) than poly-house (with humidity and temperature control). Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analysis of regenerated plants showed genetic similarity to mother plant.

Additional key words

acclimatization heat shock ISSR micropropagation RAPD sucrose 





basal MS medium supplemented with 5.0 μM BA


basal MS medium supplemented with 5.0 μM IBA


basal MS medium


heat shock


indole-3-butyric acid


inter simple sequence repeat




plant growth regulators


polymerase chain reaction


random amplified polymorphic DNA


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© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Centre of Relevance and Excellence in Agro and Industrial BiotechnologyThapar UniversityPatialaIndia

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