Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources
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The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3 −) or both nitrogen forms, nitrate and ammonium (NH4 +/NO3 −). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4 +/NO3 − in comparison with NO3 − grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4 +/NO3 − treatment. The PEG treatment decreased enzyme activities in NH4 +/NO3 − grown plants, but in NO3 − grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3 − grown plants, and lower activities of both, acidic and basic isoforms in NH4 +/NO3 −grown plants.
Additional key wordsantioxidative enzymes proline phenolics Zea mays L.
class III peroxidases
reactive oxygen species
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This work was supported by the Ministry of Science (Republic of Serbia), Projects143020B and TR-20014.
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