Biologia Plantarum

, Volume 54, Issue 3, pp 530–534 | Cite as

Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources

  • M. Vuletić
  • V. Hadži-Tašković Šukalović
  • K. Marković
  • J. Dragišić Maksimović
Brief Communication


The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3 ) or both nitrogen forms, nitrate and ammonium (NH4 +/NO3 ). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4 +/NO3 in comparison with NO3 grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline was increased, especially in NH4 +/NO3 treatment. The PEG treatment decreased enzyme activities in NH4 +/NO3 grown plants, but in NO3 grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated increased activities of acidic POD isoforms in PEG treated NO3 grown plants, and lower activities of both, acidic and basic isoforms in NH4 +/NO3 grown plants.

Additional key words

antioxidative enzymes proline phenolics Zea mays L. 



ascorbate oxidase


ascorbate peroxidase


isoelectric focusing


polyethylene glycol


class III peroxidases


reactive oxygen species


superoxide dismutase


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This work was supported by the Ministry of Science (Republic of Serbia), Projects143020B and TR-20014.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • M. Vuletić
    • 1
  • V. Hadži-Tašković Šukalović
    • 2
  • K. Marković
    • 1
  • J. Dragišić Maksimović
    • 2
  1. 1.Maize Research InstituteZemun PoljeZemunSerbia
  2. 2.Institute for Multidisciplinary ResearchBelgradeSerbia

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